芦荟大黄素的差示脉冲伏安法测定

Determination of Aloe-emodin by Differential Pulse Voltammetry

目的 建立芦荟大黄素的差示脉冲伏安测定方法。 方法 在 p H4.0的磷酸盐缓冲溶液 ,开路搅拌富集 2 min,于 0 .4～ -0 .8V电位范围用差示脉冲伏安法测定芦荟大黄素的还原峰电流。结果 在 5× 10 - 8～ 2× 10 - 7mol· L- 1 范围内 ,芦荟大黄素的差示脉冲伏安峰电流与其浓度有良好的线性关系 ,检测限为 3× 10 - 8mol·L- 1。 结论 本方法简便、快速、灵敏度高、重现性好 ,用于芦荟大黄素的含量测定 ,结果满意。

OBJECTIVE To establish a novel differential pulse voltammetric method for aloe-emodin determination.METHODS After accumulation at open circuit potential for 2min, the differential pulse voltammograms for the aloe-emodin reduction signals from +0.4 to～-0.8V were monitored in 0.1 mol·L -1 phosphate buffer solution (pH 4.0).RESULTS The peak current is proportional to the concentration of aloe-emodin over the range of 5×10 -8 ～2×10 -7 mol·L -1 .The detection limit is 3×10 -8 mol·L -1 。CONCLUSION The method is simple,rapid,sensitive and reliable for aloe-emodin analysis.