杜仲叶绿原酸的提取及精制

Extraction and purification of chorogenic acid from leaves of Eucommia ulmoides

对杜仲叶绿原酸的提取进行研究。方法 采用乙醇为溶剂 ,经正交实验方法对杜仲叶绿原酸的提取进行研究 ,并采用大孔树脂对其精制。结果 乙醇提取杜仲叶绿原酸的最佳工艺条件为 :温度 80℃ ,料液比 1∶10 ,乙醇浓度 6 0 % ,提取时间 2小时。所筛选的 NKA- 树脂是吸附绿原酸的理想吸附剂。结论 适宜于产业化生产。

Objective To observe the extraction and purification of chlorogenic acid from leaves of Eucommia ulmoides.Methods According to orthogonal design,to extract chlorogenic acid from leaves of Eucommia ulmoides with ethanol as solvent,and to purify it with resin.Results Optimal conditions for the extraction of cholorogenic acid were obtained:the leaves extracted by 60% ethanol at 80℃ for 2h with a ratio(material to solvent)of 1∶10.The selected NKA-Ⅱ resin was optimal absorbent to purify chlorogenic acid.Conclusion It was suitable to be used in industry.