复方丹参滴丸与福辛普利联用对自发性高血压大鼠左室纤维化的影响

Effect of Compound Danshen Dripping Pills Combined with Fosinopril on Left Ventricular Fibrosis of Spontaneously Hypertensive Rats

目的 探讨复方丹参滴丸 (DSP)与福辛普利联用对自发性高血压大鼠 (SHR)左室纤维化的影响。方法 将 48只8周龄雄性SHR随机分为 6组 :DSP小剂量组 ;DSP大剂量组 ;福辛普利组 ;DSP小剂量与福辛普利联用组 ;DSP大剂量与福辛普利联用组 ;SHR对照组。 6组分别用灌胃法给药8周 ,测大鼠尾动脉收缩压、左室肥厚指数 ;局部心肌 /血浆血管紧张素Ⅱ、血浆醛固酮浓度 ;通过天狼星红染色进行心肌胶原定性和半定量分析。结果 与对照组比较 ,DSP可明显减轻左室肥厚 (P <0 .0 1) ,改善左室纤维化 (P <0 .0 1或P <0 .0 5 ) ,并与福辛普利联用时可进一步提高后者的抗心肌纤维化效应 ;DSP尤其与福辛普利联用时可降低血浆AngII、Ald及局部心肌AngII浓度 (P <0 .0 1或P <0 .0 5 )。结论 DSP与福辛普利联用可改善SHR心肌纤维化(MF)。

Objective This study was to investigate effects of both compound Danshen dripping pills (DSP) and the combined use of DSP with fosinopril on left ventricular fibrosis of spontaneously hypertensive rats. Methods 48 eight-week-old male spontaneously hypertensive rats (SHRs) were assigned randomly into six groups: low dose DSP (SHRdl); high dose DSP (SHRdh);fosinopril (SHRf); combination use of low dose DSP with fosinopril (SHRdl+f); combination use of high dose DSP with fosinopril (SHRdh+f) and placebo group with distilled water (SHRc). Drugs and distilled water were given to each group for 8 weeks. Arterial systolic blood pressure (SBP) and left ventricular hypertrophy index (LVHI) were measured. Contents of angiotensin Ⅱ(AngⅡ) and aldosterone (Ald) were detected by radioimmunoassay.Myocardial collage was stained and collage volumefraction was determined in the left ventricle by quantitative morphometry. Results compare with the control group,①DSP can decrease left ventricle collage and LVHI of SHR(P<0.01 or P<0.05)and enhance the anti-left-ventricular -fibrosis effect of fosinopril.②DSP can decrease the concentration of plasma AngⅡ and Ald and the concentration of AngⅡin myocardium (P<0.01 or P<0.05). Conclusion DSP and combined use of DSP and fosinopril can decrease left ventricular fibrosis.