趋化因子受体CCR7促进乳腺癌细胞的趋化与侵袭

CC chemokine receptor-7 induces chemotaxis and invasion of breast cancer cells

目的 :研究趋化因子受体CCR7的表达对乳腺癌细胞趋化活性与侵袭活性的促进作用 .方法 :RT PCR法测定 3株乳腺癌细胞MCF 7,MDA MB 2 31,MDA MB 36 1中CCR7的表达 ;在CCR7的配体SLC作用下 ,通过趋化小室法检测不同乳腺癌细胞的趋化活性与侵袭活性 ;检测CCR7的封闭对乳腺癌细胞趋化活性和侵袭活性的影响 .结果 :3株乳腺癌细胞中均存在不同程度CCR7的表达 ,其配体SLC对 3种乳腺癌细胞均存在不同程度的趋化活性和侵袭活性 ,CCR7的封闭也能够在不同程度上抑制乳腺癌细胞的这种趋化活性和侵袭活性 .结论 :趋化因子受体CCR7的表达能够促进乳腺癌细胞的趋化与侵袭 ,从而可能在乳腺癌细胞向淋巴结转移中发挥重要作用 .

AIM: To study the chemotaxis and invasion of breast cancer cells facilitated by CC chemokine receptor-7 (CCR7). METHODS: Reverse transcription-PCR analysis of CCR7 was performed in three breast cancer cell lines. The chemotaxis and invasion assays were performed on breast cancer cells in presence of secondary lymphoid tissue chemokine (SLC), a ligand of CCR7, and when CCR7 was blocked. RESULTS: Breast cancer cell lines expressed different levels of CCR7 mRNA. SLC-mediated chemotaxis and invasion could be checked in those breast cancer cells. Both chemotaxis and invasion could be blocked by neutralizing anti-CCR7 antibody. CONCLUSION: Chemotaxis and invasion of breast cancer cells can be facilitated by CCR7 expression. CCR7 may play an important role in the lymph node metastasis of breast cancer.