携ICAM-1单抗超声造影剂体外评估兔腹主动脉内皮损伤实验研究

Detecting endothelial dysfunction of abdominal aorta in rabbits in vitro with microbubbles conjugated anti-ICAM-1

目的 探讨携ICAM 1单抗的超声造影剂评价血管内皮损伤的可行性及其价值。方法 FITC标记自制含氟碳声振白蛋白造影剂及携ICAM 1单抗的靶向超声造影剂。新西兰大白兔 8只 ,高脂饮食建立内皮损伤动物模型后 ,取兔腹主动脉做冰冻病理切片 ,随机分两组 ,分别滴加非靶向造影剂、靶向造影剂 ,比较两种微泡内皮分布情况及相应荧光染色度的差异。结果 靶向造影剂组内皮黏附微泡明显增多 ,荧光染色较强。结论 携ICAM 1单抗的造影剂微泡可靶向黏附于损伤内皮 。

Objective To discuss the value and feasibility of targeted microbubbles conjugated to anti-ICAM-1 in monitoring endothelial dysfunction. Methods Fluorescein isothiocyanate (FITC) was mixed with self-made microbubbles and anti-ICAM-1 conjugated microbubbles respectively. Eight New Zealand rabbits were fed with cholesterol diet to create the animal model of endothelial dysfunction. Aorta was dissected and tissue slides were prepared. Two kinds of microbubbles were added to the glass slide and the behaviors of targeted and nontargeted microbubbles were observed through fluoroscope. Results Microbubbels retained within the endothelium were much more for anti-ICAM-1 containing than for standard microbubbles. The fluorescent signal from the endothelium for targeted microbubbles was much higher than that of nontargeted ones. Conclusion Microbubbles conjugated to anti-ICAM-1 have been shown to bind to activated endothelial cells. It can be used to assess early endothelial damage.