优良籼型恢复系转基因植株的获得及其遗传稳定性(英文)

TRANSGENIC ELITE INDICA RESTORER LINE AND GENETIC STABILITY OF TRANSGENES

报道了用基因枪转化法将雪花莲凝集素基因 (gna)转移到优良籼型杂交稻恢复系蜀恢 5 2 7中 .PCR、PCR -Southernblotting和Southernblotting等分子检测证明 ,外源基因以多拷贝单位点方式整合到受体基因组中并稳定遗传到转基因第三代 (T2 ) .转基因第一代植株 (T0 )在株高和结实率上与相应的组培、种子实生苗植株相比 ,发生明显的不可遗传的变异 ,随着繁殖代数的增加 ,转基因植株恢复到与对照植株一致 .Westernblotting表明 ,目的基因在转基因植株中正确表达 .蛋白活性分析显示 ,外源基因在转基因植株中合成的蛋白具有凝血生物活性 ,含量占可溶性总蛋白的0 .1%左右 .本文还对转基因技术在杂交稻育种中的应用进行了讨论 .图 4表 2参

This paper reports that the transgenic elite indica restorer line containing snowdrop lectin (Galanthus nivalis agglutinin, GNA) gene gna was obtained by biolistics and identified by PCR, PCR-Southern blotting and Southern blotting that the transgenes were integrated with estimated multi-copy at the same location of the genome of SH527 and transmitted stably from the first generation (T0) of transgenic plants to the third generation (T2). The plant height and seed-setting percentage of the first generation (T0) of transgenic plants were reduced apparently as compared with the corresponding plants derived from seeds and untransformed calli. However, this kind of variation could not be inherited stably. The protein analysis showed that the insect-resistant gene expressed in T0, T1 and T2 plants. The expressed protein had the biological activity and the ratio of GNA from transgenic plants to their total soluble protein was about 0.1%. The usage of genetic engineering in the breeding programs of hybrid rice was also discussed. Fig 4, Tab 2, Ref 18