小麦铝抗性和敏感品系对铝胁迫的生理生化反应

PHYSIOLOGICAL AND BIOCHEMICAL RESPONSE OF Al-SENSITIVE AND Al-TOLERANT WHEAT TO ALUMINUM

研究了冬小麦TriticumaestivumL .铝抗性品系T2 0 2和铝敏感品系T10 5幼苗经 0 ,5 0 μmolL-1,75 μmolL-1,10 0 μmolL-1和 15 0 μmolL-1铝处理后根部的生理生化变化 .结果表明 :小麦根生长被严重抑制 ;酸性磷酸酶活性增强 ,磷代谢激活 ;硝酸还原酶受到一定程度的抑制 ;葡萄糖 - 6 -磷酸脱氢酶对铝浓度极其敏感 ;葡萄糖含量下降 ;而膜脂过氧化产物丙二醛 (MDA)含量上升 ,超氧化物歧化酶 (SOD)的活性升高和还原型谷胱甘肽 (GSH)的含量下降 .证实 :铝胁迫造成了氧化伤害 ,激活了植物体内的抗氧化系统 .分析还表明 ,T2 0 2耐铝的各项指标优于T10 5 ,前者的代谢酶活性对铝胁迫的反应更为迅捷 ,并有良好的能量储备和调动机制来抵御铝胁迫及由此产生的胁迫 .图 8参

The physiological and biochemical responses of Al-tolerant cultivar T202 and Al-sensitive cultivar T105 of winter wheat, Triticum aestivum L., were investigated under exposing to aluminum concentration of 0, 50 μmol L -1, 75 μmol L -1, 100 μmol L -1 and 150 μmol L -1. The results showed that the growth of wheat root was inhibited seriously, the activity of acid phosphatase was enhanced, the activity of nitrate reductase was inhibited to some extent, G6PDHase was very sensitive to aluminum stress and the content of glucose decreased with aluminum concentration increased. Furthermore, the increment of malondialdehyde (MDA) level and the enhancement of SOD activity as well as the decrease of GSH content definitely indicated that aluminum stress could cause oxidative injury and activate the antioxitant system in wheat. T202 demonstrated a better aluminum-tolerance than T105 through that the enzymes in T202 responded more promptly towards aluminum stress and that T202 possessed higher energy pool as well as more effective mechanism to resist aluminum stress and the subsequent oxidative stress. Fig 8, Ref 14