MUC1的表达与乳腺癌细胞粘附的关系

Correlation of MUC1 Expression to Adhesion of Breast Cancer Cell Line MDA-MB-231

背景与目的:近年研究认为,肿瘤的转移与肿瘤细胞膜上粘液型糖蛋白的大量形成有关。本实验研究糖基化抑制剂苯甲基-α-N-乙酰半乳糖胺对粘蛋白-1(mucin1,MUC1)形成的抑制作用及其对乳腺癌细胞株MDA-MB-231细胞的粘附、侵袭转移能力的影响。方法:免疫细胞化学法检测MUC1表达,噻唑蓝比色法(MTT)检测细胞对人工基底膜(Matrigel)的粘附能力,明胶酶谱法(zymography)检测MDA-MB-231细胞MMP-2、MMP-9的分泌变化。结果:经苯甲基-α-N-乙酰半乳糖胺去糖基化处理后的肿瘤细胞组与相应对照组比较,MUC1表达降低,对基底膜的粘附力明显降低(P<0.01),同时细胞中MMP-2和MMP-9的分泌量显著下降。结论:MUC1表达水平与肿瘤细胞的粘附能力相关,MUC1的抑制使乳腺癌MDA-MB-231细胞粘附能力,分泌Ⅳ型胶原酶能力明显减弱。

BACKGROUD &OBJECTIVE:Recent researches found that an abundant production of mucin protein well correlates with tumor cell metastasis. This study was to investigate inhibitory effect of benzyl α GalNAc on production of mucin 1 (MUC1), and on adhesion and invasion of breast cancer cell line MDA MB 231. METHODS:MDA MB 231 cells were incubated with benzyl α GalNAc, expression of MUC1 was detected by immunohistochemistry, adhesive ability of MDA MB 231 cells to artificial basement membrane Matrigel was measured by MTT assay. Gelatin zymography was performed to detect the secretion changes of matrix metalloproteinase 2 (MMP 2) and MMP 9. RESULTS:Compared with control cells, the tumor cells deglycosylated by benzyl α GalNAc showed lower expression of MUC1 (P < 0.05), and less adhesion to the Matrigel (P< 0.01), the secretion of MMP 2 and MMP 9 suppressed (P< 0.05). CONCLUSION:The expression of MUC1 correlates to adhesion and invasion of MDA MB 231 cells, benzyl α GalNAc may weaken adhesion and type Ⅳcollagenase secreting activity of MDA MB 231 cells by inhibiting MUC1.