肿瘤基因组学研究中纯化技术的应用与发展

Application and Development of Purification in Cancer Genomics Research

为了获得尽可能纯净的肿瘤组织、细胞用于肿瘤基因组学研究,人们发展了一系列的肿瘤组织纯化技术。从各种手工显微切割纯化技术,到借助各种机械手段并经显微切割纯化肿瘤组织,再发展到激光技术在组织纯化领域内的全面应用,推动了肿瘤基因组学研究的发展。每一种纯化技术都有其各自的优势与不足,究竟采取哪种方式,取决于研究者对实验的要求以及研究者所处的环境条件。

In the study of cancer genomics, a series of purification methods have been invented to collect homogenous cells from heterogenous cancerated tissue. Tissue microdissection methods, such as manual microdissection, mechanical microdissection, and laser technology, accelerated cancer genomics research. Each method holds both advantage and disadvantage, the selection of methods depends on the demands of study, and the instruments used in study.