赤芍总苷对培养乳鼠心肌细胞损伤的抗氧化作用

Antioxidative effect of total paeony glycoside on cultured neonatal rat cardiomyocytes injured

目的 探讨赤芍总苷对心肌细胞损伤的抗氧化保护作用。方法 以培养的乳鼠心肌细胞加入异丙基肾上腺素造成缺血缺氧损伤模型 ,对比分析正常对照组、药物损伤组、辅酶Q10 阳性对照组、以及不同剂量赤芍总苷保护组培养液中超氧化物歧化酶、丙二醛和一氧化氮含量。结果 损伤组总SOD、CuZn SOD和Mn SOD水平均明显下降 ,MDA和NO含量显著升高 ;而辅酶Q10 组和赤芍总苷组上述指标都有不同程度的改善 ,其中大剂量赤芍总苷组的保护作用接近或优于阳性对照组。结论 赤芍总苷对异丙基肾上腺素造成的心肌损伤具有保护作用 ,且呈现剂量依赖关系 ,作用机制与增强细胞抗氧化作用 ,减少自由基和脂质过氧化物导致的细胞膜损伤有关。

Aim To investigate the antioxidative effect of total paeony glycoside(TPG) on cardiomyocytes injured.Methods The ischemia and hypoxia injuy model of cultured neonatal rat cardiomyocytes was induced by adding isoprenaline(ISO), and superoxide dismutase(SOD), malonalde hyde(MDA) and nitric oxide(NO) in the culture solution of normal control group; ISO injure group, CoQ 10 positive control group as well as protective group s with high,middle or low-dose TPG were respectively analyzed and compared. Results Compared with normal control group,the enzyme activity o f total SOD, CuZn-SOD and Mn-SOD decreased obviously, and the content of MDA and NO increased markedly in injury group, but in TPG and CoQ 10 groups all of detective indicators had improvement in varying degrees, and the protective effect was better than or close to positive control group in high-dose TPG grou p.Conclusion TPG has protective action on injured cardiomyocyte s induced by ISO in dose-dependent manner. The mechanism relates to the enhance ment of antioxidative effect in cells, and the reduction of membrane damage caus ed by free radical and lipid peroxide.