D-半乳糖对不同性别大鼠骨形态计量学的影响

The different effects of D-galactose onbone histomorphometry in different gender rats

目的 探讨D 半乳糖对大鼠骨形态计量学的影响。方法 3月龄SD大鼠 4 2只♀♂各半 ,按体重随机分成 5组 ,对照、去势和D 半乳糖低 (5 0mg·kg-1·d-1)、中 (10 0mg·kg-1·d-1)、高 (2 0 0mg·kg-1·d-1)剂量 5个组 ,前两组分别皮下注射生理盐水、后三组分别皮下注射三个不同浓度的D 半乳糖。两个月后心脏取血处死老鼠 ,取出左侧股骨和胫骨 ,胫骨脱水塑料包埋切片 ,用骨形态计量学方法测量骨的静态参数和动态参数 ,股骨称其干重。处死大鼠前用双荧光标记法标记。同时取出卵巢和睾丸制作软组织石蜡切片 ,在显微镜下观察。结果 D 半乳糖对♀大鼠骨没有明显影响 ,但可使♂大鼠骨明显丢失 ,呈现骨质疏松的形态学表现。♂大鼠三个剂量组中 ,与骨量有关的静态参数骨小梁百分率 (%Tb .Ar)分别下降 5 3 5 %、5 3 5 %、4 6 6 % ,骨小梁数目 (Tb .N)分别降低 4 2 9%、4 7 6 %、38 1% ,骨分离度 (TB .Sp)分别升高85 %、15 0 %、6 7 3%。与骨形成有关的动态参数骨骨形成标记百分率 (%L .Pm)分别下降 2 4 1%、5 3 5 %、4 6 6 %。卵巢组织切片未见明显变化 ,睾丸组织曲细精管排列紊乱 ,管腔增大 ,有的甚至基膜溶解。结论 D 半乳糖对大鼠骨形态学的影响有性别差异 ,对♀大鼠没有影响 ,可致♂大鼠骨质疏松 ,可能?

Aim To study the effects of D-galactose o n bone of rats. Methods Forty-two Sprague-Dawley rats at age of 3 months with half female and half male were divided into five groups randoml y, basal, OVX or ORX , D-galactose at dose of 50 mg·kg -1·d -1 (L), D-galactose at dose of 100 mg·kg -1·d -1 (M), galactose a t dose of 200 mg·kg -1·d -1 (H).The former two groups were SC inject ed physiological saline and the latter three groups were injected three differen t doses of D-galactose respectively. Two months later the rats were killed by setting blood free from hearts, then the left thighbones and tibias were take n. The tibias were cut at 8 μm sections for static and dynamic bone histomorph ometric analyses. The thighbones were dried and weighed. All rats were given sc injections of tetracycline 25 mg·kg -1 (first fluorochrome bone marker) on 14 and d 13,and calcein 10 mg·kg -1(second fluorochrome bone marker) on 4 and d 3 before sacrifice. The testicles and the ovaries were taken and made the paraffin slices for observing the microstructure under the microscope. Results D-galactose had no effects on bone of female rats, but can made male rats lose bone significantly and the rats appeared osteoporosis. The static parameters in three different dose male rats bone which relate to bone ma ss %Tb. Ar decreased 53.5%,53.5%,46.6% respectively, Tb.N decreased 42.9%,4 7.6%,38.1% respectively, TB.Sp increased 85%,150%,67.3% respectivly. The dynamic parameters which relate to bone formation %L. Pm decreased 24.1% 89.1% , 65.5% respectively. The convoluted suminiferous tubule disordered, lumens dil ated, and basal membrane disintegrated. Conclusion D-galac tose had different effects on bone of different gender rats. There is no effect on female bone, but it can induce the male rats osteoporosis. The mechanism of D-galactose on bone was probably due to the different effects on gonad glan ds.