缺氧诱导因子1α及其相关基因在腹主动脉瘤中的表达

The Expression of Hypoxia-Inducible Factor-1α and Related Genes in Abdominal Aorta Aneurysm

为了研究缺氧诱导因子 1α及其相关基因在腹主动脉瘤中的表达 ,选取腹主动脉瘤标本 2 2例 ,以 5例正常腹主动脉作对照 ,检测缺氧诱导因子 1αmRNA及蛋白的表达 ,检测血管内皮生长因子及促红细胞生成素的表达及微血管密度。结果发现 ,腹主动脉瘤组织中缺氧诱导因子 1αmRNA及蛋白表达明显高于正常腹主动脉 (P <0 .0 1) ;血管内皮生长因子和促红细胞生成素表达亦明显增强 (P <0 .0 1)。缺氧诱导因子 1α表达主要分布在腹主动脉瘤中层血管平滑肌细胞及外膜处 ,与血管内皮生长因子和促红细胞生成素的分布部位基本一致。腹主动脉瘤中微血管密度明显增加 (P <0 .0 1) )。结果提示 ,缺氧诱导因子 1α在腹主动脉瘤发病过程中可能发挥重要作用 。

Aim To study the expression of hypoxia-inducible factor-1α (HIF-1α) and related genes in abdominal aorta aneurysm (AAA). Methods 22 AAA specimens were collected and 6 normal abdominal aorta tissue were used as control. Northern blot was used to evaluate the expression of HIF-1α mRNA. Western blot and immunohistochemistry method was used to determine the expression of HIF-1α, vascular endothelial growth factor (VEGF) and erythropoietin (EPO). Microvessel density(MVD) was also studied. Results The expression of HIF-1α was significantly higher in AAA than that in normal abdominal aorta (P<0.01). The expression of VEGF and EPO were also higher in AAA (P<0.01). Most of the positive cells located in VSMC and adventitia of AAA. The MVD counts were higher in AAA. Conclusion HIF-1α may have an important role in the onset of AAA, which may be obtained by promoting the expression of VEGF and EPO.