沥水调脂胶囊对轻度修饰低密度脂蛋白诱导的血管平滑肌细胞单核细胞趋化因子1及内皮细胞P选择素表达的影响

Effects of Li Shui Tiao Zhi Capsule on Monocyte Chemoattractant Protein-1 of Vascular Smooth Muscle Cells and P-Selectin of Vascular Endothelial Cells Induced by Minilly Modified Low Density Lipoprotein

探讨健脾祛痰化瘀方药 (沥水调脂胶囊 )含药血清对轻度修饰低密度脂蛋白诱导的血管平滑肌细胞表达单核细胞趋化因子 1及内皮细胞表达P选择素的影响。用细胞酶联免疫法测定内皮细胞P选择素的表达 ,酶联免疫法测定培养血管平滑肌细胞上清液中单核细胞趋化因子 1的含量。结果发现 ,2 0 %沥水调脂胶囊除蛋白含药血清组对轻度修饰低密度脂蛋白诱导的血管平滑肌细胞单核细胞趋化因子 1表达的升高具有一定的抑制作用。10 %含药血清组对轻度修饰低密度脂蛋白诱导的内皮细胞表达P选择素已具有抑制作用 ,当含药血清浓度达 2 0 %时其抑制作用更为明显。由此可见 ,通过抑制细胞炎性因子单核细胞趋化因子 1和血小板活化因子P选择素 ,减轻血管炎性反应和血栓形成 ,是健脾祛痰化瘀方药沥水调脂胶囊抗氧化损伤。

Aim To explore the effects of Li Shui Tiao Zhi Capsule (LSTZ) on monocyte chemoattractant protein-1 (MCP-1) of vascular smooth muscle cells (VSMC) and P-selectin of vascular endothelial cells (EC) induced by minilly modified low density lipoprotein (mm-LDL). Methods Cells were treated by different concentrations of serum-containing LSTZ (5%,10% and 20%) and mm-LDL. MCP-1 of VSMC was determined with ELISA. P-selectin of EC was detected with Cell-ELISA. Results The expression of MCP-1 induced by mm-LDL (20 mg/L) can be reduced by 20% serum-containing LSTZ (P<0.05). The expression of P-selectin induced by mm-LDL (40 mg/L) was suppressed by 10% serum-containing LSTZ (P<0.05), and it showed a notably inhibition on concentration of 20% (P<0.01). Conclusions Protecting vascular cell and relieving respone of inflammation may be one of the mechanisms of anti-atherosclerosis of LSTZ. Not only MCP-1 but also P-selectin may be involved in the “XUEYUZHENG' in Chinese Medical theory.