脂肪分化相关蛋白在兔动脉粥样硬化模型中的表达

Expression of Adipophilin in the Rabbit Atheroscleritic Model

研究脂肪分化相关蛋白与动脉粥样硬化发生和发展的关系。使用高胆固醇饲料喂养新西兰白兔 12周 ,复制动脉粥样硬化模型。用生物化学法测定血清总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、甘油三酯及动脉壁胆固醇含量 ;苏丹Ⅳ染色观察主动脉斑块面积 ;HE染色观察主动脉及肝脏的病变 ;免疫组织化学法观察脂肪分化相关蛋白在主动脉病变区及肝脏中的表达。结果发现 ,喂高胆固醇饲料的动物血清总胆固醇、低密度脂蛋白胆固醇、甘油三酯及动脉壁胆固醇含量显著升高 ,主动脉病变面积为 4 0 .1%± 7.3% ,脂肪分化相关蛋白在主动脉动脉粥样硬化病变区免疫组织化学染色呈强阳性 ,在肝脏中染色呈阴性。喂高胆固醇饲料能成功复制出兔动脉粥样硬化模型。脂肪分化相关蛋白在兔动脉粥样硬化病变中表达明显增高 。

Aim To explore the relationship between adipophilin and atherosclerosis. Methods The New Zealand white rabbits were fed with high cholesterol chow for 12 weeks and became the model of atherosclerosis. The level of serum total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, triglyceride, and cholesterol content of aortic wall were investigated. The areas of fatty streak of the aortae were measured after staining with Sodan Ⅳ. The aortic, and live specimens with HE and immunohistochemstry staining were observed with light microscopes. Results In the animals fed with high cholesterol chow, the level of serum total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, triglyceride, and cholesterol content of aortic wall significantly increased. The areas of fatty streak in the aortae was 40.1%±7.3% at the end of 12 weeks. The fatty streak of the aorta with immunohistochemstry staining was strongly positive, and the liver was negative with or without high cholesterol chow. Conclusions The New Zealand white rabbits could be made the model of atherosclerosis through feeding with high cholesterol chow for 12 weeks. Expression of adipophilin in atherosclerotic rabbits increased,which suggested the adipophilin was related to the development of atherosclerosis.