诱导型环氧合酶在人冠状动脉粥样硬化组织中的表达分布

Distribution of Induced Cyclooxygenase Expression in Atherosclerotic Coronary Artery

为探讨诱导型环氧合酶在人冠状动脉不同动脉粥样硬化病变类型间的分布。收集 15例尸检的人冠状动脉共 4 5个标本 ,根据HE染色病理特征及AHA标准分为 3组 ,其中正常冠状动脉对照 3个 ;纤维斑块或Ⅴ型病变(稳定斑块 )标本 2 2个 ;粥样硬化斑块伴有不同程度炎症反应的复合斑块或Ⅵ型病变 (不稳定斑块 )冠状动脉标本2 0个。分别采用免疫组织化学染色和逆转录聚合酶链反应方法 ,检测诱导型环氧合酶蛋白和mRNA在以上不同类型标本中的分布表达。结果发现 ,诱导型环氧合酶主要表达分布于动脉粥样硬化组织斑块区 ,在复合斑块中诱导型环氧合酶的表达明显高于纤维斑块 (诱导型环氧合酶灰度值 14 7.0± 1.1比 5 6 .2± 4 .4 ,P <0 .0 1) ,而在非斑块区和正常冠状动脉中均未检测到诱导型环氧合酶的表达。结果提示 ,诱导型环氧合酶参与了动脉粥样硬化病理发生以及斑块稳定性中炎性反应的调控过程 ,抑制斑块局部诱导型环氧合酶的高表达 。

Aim To evaluate the distribution of induced cyclooxygenase (COX-2)expression in human coronary arteries with different atherosclerotic lesions. Methods 45 coronary arteries specimens from 15 autopsy cases were divided into 3 groups according to pathologic features of HE staining and AHA definition respectively,which were 3 healthy coronary arteries as control,22 fibrotic plaque or type Ⅴ lesion specimens (representing stable plaque),and 20 complicated plaque or type Ⅵ lesion specimens (representing unstable plaque). RT-PCR were performed for mRNA analysis,and immunohistochemical staining were used for location of protein. Results COX-2 was found in the areas of plaques but not in healthy or nonatherosclerotic vascular walls. COX-2 expression in complicated plaques was significantly higher than in fibrous plaques (COX-2 expression 147.0±1.1 vs 56.2±4.4,P<0.01). Conclusions These findings suggests that COX-2 might play a role in pathogenesis of atherosclerosis,and modulation of inflammatory process involved in plaque stability. Inhibition of COX-2 over-expression in plaque areas might be a potential approach to atherosclerosis treatment and plaque stabilization.