葡萄籽原花青素降低动脉粥样硬化兔血清C反应蛋白水平

Effect of Grape Seed Proanthocyanidin Extract on Serum C-reactive protein in Rabbits

探讨葡萄籽原花青素对动脉粥样硬化兔血清C反应蛋白的作用。 15只雄性新西兰大白兔随机分为正常对照组 (喂饲标准颗粒饲料 )、高脂模型组 (喂饲含 1%胆固醇的颗粒饲料 )和葡萄籽原花青素组 (饲喂含 1%胆固醇和 1%葡萄籽原花青素的颗粒饲料 )。于实验开始前 1天和实验后第 1、2、4、8、12周末取空腹血 ,检测血清C反应蛋白水平。在 12周末 ,所有大白兔经空腹取血后处死 ,取其主动脉进行病理学分析。结果发现 ,与高脂模型组比较 ,葡萄籽原花青素组血清C反应蛋白水平在 1周末即明显降低 (P <0 .0 0 1) ,并维持至实验结束。高脂模型组和葡萄籽原花青素组血清C反应蛋白水平与时间均呈现高度相关性 (r =0 .990 4 7,P <0 .0 0 1和r =0 .74 777,P <0 .0 0 1)。病理学分析发现 ,葡萄籽原花青素组主动脉动脉粥样硬化病变程度 (主动脉壁厚度和泡沫细胞数量 )比高脂模型组明显降低 (P <0 .0 1)。结果提示 ,葡萄籽原花青素具有抗动脉粥样硬化作用 。

Aim To observe the effect of grape seed proanthocyanidin extract (GSPE) on serum C-reactive protein (CRP) in rabbits. Methods 15 New Zealand male rabbits were randomized into three groups with 5 rabbits in each group. The control group was fed with standard diet. The cholesterol group was fed with standard diet containing 1% cholesterol. The GSPE group was fed with standard diet containing 1% GSPE plus 1% cholesterol. All the rabbits were fed for twelve weeks. Blood samples were drawn from ear middle arteries of rabbits just before the experiment and at the first, the second, the forth, the eighth and the twelfth weekend of the experiment. All the rabbits were fasted for at least eight hours before the blood was drawn. Serum CRP was determined using a commercially available CRP EIA kit. All the rabbits were sacrificed at the twelfth weekend and aortas of the rabbits were excised for the morphological observation. Aorta samples were observed through optical microscope. Results Compared with the cholesterol group, the serum CRP contents were lowered markedly by dietary GSPE (P<0.001) at the first weekend and lasted to the end of the experiment. Both the cholesterol group and the GSPE group showed high correlation of the treat and time (r=0.99047, P<0.001 and r=0.74777, P<0.001). The extent and severity of atherosclerosis (the thick of aortas and the number of foam cells) of the GSPE group were less than those of the cholesterol group (P<0.01). Conclusions GSPE had the efficacy of anti-atherogenesis, one mechanism of which was that it can inhibit the CRP.