结缔组织生长因子在自发性高血压大鼠肾脏的表达及意义

Expression of Connective Tissue Growth Factor in the Kidney of Spontaneously Hypertensive Rats

为了研究结缔组织生长因子在自发性高血压大鼠肾脏中的表达及其与高血压肾损害的关系 ,以同龄雄性WKY大鼠和自发性高血压大鼠为研究对象 ,测量 12周和 32周时尾动脉压、肾功能和尿 β2微球蛋白 ,并用免疫组织化学法检测结缔组织生长因子在肾脏中的表达。结果发现 ,与同龄WKY大鼠比较 ,自发性高血压大鼠的尿素氮和血肌酐的差异无显著性统计学意义 ,而尾动脉压明显增高 (12周时前者为 138± 17mmHg ,后者为 16 9± 6mmHg ,P <0 .0 1;32周时前者为 133.4± 14 .2mmHg ,后者为 185 .7± 12 .3mmHg ,P <0 .0 1) ;尿β2微球蛋白也显著增高 (12周时前者为 4 8.1± 9.3ng/L ,后者为 76 .4± 11.2ng/L ,P <0 .0 5 ;32周时前者为 6 1.5± 14 .7ng/L ,后者为 135 .2± 2 7.6ng/L ,P <0 .0 5 ) ,且在自发性高血压大鼠组 ,32周龄大鼠的尿 β2微球蛋白也比 12周龄的明显增加 (P <0 .0 5 )。免疫组织化学半定量检测发现 ,自发性高血压大鼠组肾脏中结缔组织生长因子的表达相对与同龄WKY大鼠明显升高 (12周时前者为 0 .4 9± 0 .0 7,后者为 0 .2 8± 0 .0 5 ,P <0 .0 5 ;32周时前者为 0 .81± 0 .0 8,后者为 0 .32± 0 .0 4 ,P <0 .0 1)。同样在自发性高血压大鼠组 ,结缔组织生长因子在 32周龄大鼠肾脏的表达也比 12周龄?

Aim To investigate the expression of connective tissue growth factor (CTGF)in the kidney of spontaneously hypertensive rats. Methods The comprised normotensive control (WKY)and hypertensive groups(SHR) were taken as the objects of study,of which blood pressure,urinary β2-microglobulin,Bun,Scr were acquired at 12-week old and 32-week old respectively,at the same time,the renal expression of CTGF were determined,when the rat were sacrificed. Results In SHR,the blood pressure,urinary microglobulin,except Bun,Scr were significantly increased compared with age-matched controls,even in the SHR group,the urinary β2-MP in 32-week old group were markedly increased,compared with that in 12-week old group. Semi-quantitative assessment of the immune-histochemical staining showed that renal expression of CTGF in SHR groups were significantly increased compared with that in WKY groups. Futher more,in SHR,the expression of CTGF in 32-week old group were also markedly increased compared with that in 12-week old group,and it is positively corelated with the increasing of urinary β2-microglobulin. Conclusion Renal expression of CTGF is increased in SHR,which is possibly involved in the development of renal damage.