明胶改性聚β-羟基丁酸酯与鼠肾上腺细胞的生物相容性

Biocompatibility of PHB Modified by Gelatin to Adrenocortical Cells of Rats

目的 :探索明胶改性的聚 β-羟基丁酸酯 (PHB)与鼠肾上腺细胞的生物相容性 ,为以PHB为细胞载体进行肾上腺细胞移植提供实验依据。方法 :以明胶为改性材料对PHB进行改性 ,并将材料与肾上腺细胞共同培养 ,通过观察PHB与明胶对肾上腺细胞的形态学、增殖 (MTT法 )与分泌功能 (放免法 )的影响 ,来评价明胶改性的PHB与鼠肾上腺细胞的生物相容性。结果 :5 %浓度的明胶改性效果较好 ;明胶改性的PHB内可见细胞呈团簇状聚集生长 ,未改性的PHB内无细胞生长 ;PHB及改性材料明胶对体外培养的肾上腺细胞增殖活性 (1.87± 0 .0 3vs1.91± 0 .0 4 ,P >0 .0 5 )以及分泌功能均无影响 [(36 .4 3± 3.6 5 ) pg·ml-1vs (31.72± 2 .4 9) pg·ml-1,P >0 .0 5 ]。结论 :明胶可增加PHB的亲水性能 ;PHB及明胶对肾上腺细胞的生长、增殖、分泌功能无影响 ;以PHB为载体的肾上腺细胞移植治疗肾上腺皮质功能不全具有临床可行性。

Objective: To obtain the experimental basis for carring out the transplantation of adrenocortical cells using poly-β-hybroxybutyric acid (PHB) as cell carrier. Methods: Adrenocortical cells were cultured with PHB modified by gelatin, and the effects of PHB and gelatin on adrenocortical cells from rats in the aspects of morphology, proliferation and secreting function were evaluated by microscopy, MTT and RIA methods. Results: The satisfactory modified results of PHB were getted by 5% gelatin. The cluster proliferating adrenocortical cells were observed in PHB modified by gelatin, with no survival adrenocortical cells in no-modified PHB. There were no effects of PHB and gelatin on adrenocortical cells from rats in the aspects of proliferating(1.87±0.03 vs 1.91±0.04,P>0.05) and secreting function(36.43±3.65 pg·ml -1 vs 31.72±2.49 pg·ml -1,P>0.05). Conclusion: Gelatin could improve the affinity of PHB to water. The modified PHB is a kind of ideal biodegradable material as cell carrier for transplantation of adrenocortical cells.