硒对氟诱导的大鼠口腔粘膜细胞和肝细胞DNA损伤的作用

Effects of Selenium on Oral Mucosal Cell and Hepatocyte DNA Damage Induced by Fluorosis in Rats

目的 :研究硒对氟中毒诱导的大鼠口腔粘膜细胞和肝细胞DNA损伤的作用。方法 :选用雄性SD大鼠 ,氟中毒组大鼠饮用含 15 0mg·L-1氟化钠 (NaF)的蒸馏水溶液 ,硒氟联合作用组大鼠饮用含 15 0mg·L-1氟化钠(NaF)和 2mg·L-1亚硒酸钠 (Na2 SeO3 )的蒸馏水溶液 ,染毒 4周 ,用单细胞凝胶电泳检测细胞DNA损伤。结果 :氟中毒能明显诱导大鼠口腔粘膜细胞和肝细胞的DNA损伤 ,总损伤细胞百分率分别为 5 0 .2 0 %和 4 4 .80 % ,而硒对氟造成的口腔粘膜细胞和肝细胞DNA损伤具有明显的拮抗作用 ,总损伤细胞百分率分别为 14 .6 0 %和 12 .6 0 %。结论 :硒对氟中毒引起的大鼠口腔粘膜细胞和肝细胞DNA损伤具有明显的拮抗作用。

Objective: To study the effects of selenium on oral mucosal cell and hepatocyte DNA damage induced by fluorosis in rats.Methods: Three groups of male SD rats were provided with deionized drinking water containing 0(control group), 150 mg·L -1 sodium fluoride(fluorosis group), 2 mg·L -1 sodium selenite and 150 mg·L -1 sodium fluoride(combinative action group of selenium and fluoride) respectively for 4 weeks. Single cell gel-electrophoresis assay was used to detect the DNA damage rate in rat oral mucosal cells and hepatocytes. Results: The DNA damage rates of fluorosis group were 50.20% in oral mucosal cells and 44.80% in liver cells and were higher than those of the control group significantly. The results also showed that selenium had antagonistic effect on DNA damage induced by fluorosis and could decrease the rates of DNA damage to 14.60% in oral mucosal cells and 12.60% in liver cells respectively. Conclusion: It suggests that selenium at certain concentration relieves DNA damage induced by fluorosis in rat oralmucosal cells and hepatocytes in vivo.