二色补血草多糖的结构表征及其对Hela细胞的抑制作用

Isolation and Structural Analysis of the Polysaccharides of Limonium bicolor and the Inhibition to Hela Cell

从中药二色补血草中分离提取到一种水溶性多糖 ( LP) . 1 H NMR,1 3C NMR,IR,HPLC和 GC-MS等方法分析表明 ,LP是以α-D-[Glc A( 1→ 6) Glc]二糖为结构单位 ,每个重复的二糖单位彼此以α-( 1→ 4)糖苷键连接成直链多糖 .荧光光谱显示 LP的荧光谱峰出现在 460 nm附近 ,Zn2 + ,Ca2 + 通过与 LP络合 ,其荧光强度增加 .MTT实验表明 ,LP具有抑制肿瘤细胞活性 ,对 Hela细胞抑制的 IC50 为 62 .2 μg/m L.

Soluble polysaccharides were isolated from Limonium bicolor Kunge(Bge.) and purified by conventional method. IR, GC-MS, 13C NMR and 1H NMR were used to analyze the structures of the polysaccharides. The result showed that glucose and glucose acid were the main components of the polysaccharides, and the bond between GlcA and Glc was α-(1→6), then the disaccharide unit was connected by \{α-(1→4\}) linkage. The polysaccharides were fluorescent with the biggest emission peak at 460 nm, and Zn 2+ or Ca 2+ enhanced the fluorescence intensity of LP. Much more research proved that LP had the function of inhibiting the growth of Hela cells, with the IC 50 being 62.2 μg/mL.