抑癌基因PTEN在鼻咽癌组织中的表达及意义

Expression and significance of PTEN in nasopharyngeal carcinoma

目的 :检测抑癌基因PTEN在鼻咽癌组织的表达缺失情况 ,探讨PTEN与鼻咽癌发生发展之间的关系。方法 :采用免疫组织化学SABC染色法观察鼻咽癌旁正常组织和鼻咽癌组织中PTEN蛋白的表达 ,并分析其与病理组织类型、病理分级和临床分期的关系。结果 :PTEN在鼻咽癌旁正常组织的表达缺失率 (13.3% )低于鼻咽癌组织的表达缺失率 (47.8% ) ,其差异有统计学意义 (P <0 .0 5 ) ;鼻咽鳞状细胞癌PTEN的表达缺失率(5 8.1% )高于分化型和非分化型非角化癌 (30 .8% ) ;高、中、低分化的鼻咽鳞状细胞癌中 ,PTEN的表达缺失率依次递增 (分别为 2 5 .0 %、6 3.2 %、83.3% ) ;鼻咽癌临床Ⅲ～Ⅳ期的PTEN的表达缺失率 (78.1% )高于临床Ⅰ～Ⅱ期 (2 1.6 % )。结论 :PTEN在鼻咽癌的发生及发展过程中有表达缺失 ,且与病理类型、病理分级、临床分期有关。

Objective:To investigate the expression of tumor suppressor gene PTEN in nasopharyngeal carcinomas and the relationship between PTEN and the progression of nasopharyngeal cancer.Method:The SABC immunohistochemistry method was employed to detect the expression of PTEN in paraffin-embedded nasopharyngeal carcinoma tissues. The relationship between the geneexpression and clinicopathological features was analyzed.Result:Loss of PTEN expression in normal cases (13.3%) was lower than that in nasopharyngeal carcinoma cases(47.8%). Of nasopharyngeal cancer cases, loss of PTEN expression in squama cell nasopharyngeal(58.1%) was higher than that in others tissue type(30.8%); loss of PTEN expression in squama cell nasopharyngeal had correlation with pathological stage, which increased gradually from poorly differentiated to well differentiated squama cell nasopharyngeal; loss of PTEN expression of clinical Ⅲ～Ⅳstage(78.1%) was higher than that of clinical Ⅰ～Ⅱstage(21.6%).Conclusion:It is presumed that PTEN gene deletion may be involved in carcinogenesis and progression of nasopharyngeal carcinoma, and relates to the tissue type,the pathological grade and clinical stage.