微血管密度和基质金属蛋白酶-9的表达与胶质瘤病理分级的关系

Relationship between microvascular density, expression of matrix metalloproteinase-9 and clinical pathology in glioma

目的 探讨微血管密度 (MVD)和基质金属蛋白酶 9(MMP 9)的表达与人脑胶质瘤恶性程度及预后的关系。方法 采用免疫组织化学SP法检测 5 8例不同病理分级的人脑胶质瘤及 10例正常脑组织标本中MMP 9和CD3 4 的表达 ,测定其阳性细胞数和阳性血管数。结果 胶质瘤中MMP 9表达于肿瘤细胞胞浆和血管内皮细胞 ;不同病理分级胶质瘤中MMP 9阳性表达率分别为Ⅰ级 4 2 .9% ,Ⅱ级 6 5 .0 % ,Ⅲ级 86 .7% ,Ⅳ级 88.9% ,显著高于正常脑组织 (P<0 .0 1) ,且与胶质瘤恶性程度呈正相关 (rs=0 .5 97,P <0 .0 5 ) ;不同病理分级胶质瘤中MVD存在显著性差异 (H =4 7.86 5 ,P <0 .0 5 )。结论 MVD和MMP 9的表达与胶质瘤恶性程度密切相关 ,可作为临床判断胶质瘤恶性程度、侵袭性及预后的重要指标。

Objective To explore the relation between micro va scular density (MVD), expression of matrix metalloproteinase-9 (MMP-9) and mal ignancy in glioma. Methods The expression of MMP-9 and CD 34 in 10 cases of normal brain tissues and 58 cases of glioma were detecte d by immunohistochemical streptavidin-peroxidase technique. The positive cells of MMP-9 and the positive microvessels were examined under binocular light micr oscope. Results ① The positive expression of MMP-9 in gli oma was located in the tumor cytoplasm and endothelial cells. ② The positive ra tes of MMP-9 in glioma of grades Ⅰ,Ⅱ,Ⅲ and Ⅳ were 42.9%, 65.0%, 8 6.7% and 88.9%. The expression of MMP-9 was correlated with glioma malign ancy (r s= 0.597, P< 0.05). ③ MVD was correlated with gliom a malignancy (H= 47.865, P<0.05). Conclusion The expression of MMP-9 and MVD is correlated with glioma malignancy, which may be helpful in judging the malignancy, invasion and prognosis of glioma.