健脾益气中药的小肠隐窝细胞药理靶点探讨

Pharmacological Target of Spleen-Strengthening and Qi-Invigorating Herbal Medicine on Small Intestinal Crypt-like Cells

综述近年来开展IEC-6小肠隐窝细胞药理研究的成果。研究工作以隐窝细胞生理学研究进展作为基础,建立IEC-6细胞药理实验模型,开展健脾益气中药的肠上皮干细胞药理研究。实验采用噻唑蓝比色法(MTF)、逆转录聚合酶链反应(RT-PCR)、酶联免疫吸附测定(ELISA)、高效液相色谱、免疫荧光标记等方法,观察健脾益气中药对IEC-6细胞增殖、迁移及分化的影响并观察其对绒毛蛋白、二价金属转运蛋白、白细胞介素6(IL-6)和鸟氨酸脱羧酶(ODC)的表达及对ODC活性及腐胺含量的影响,结果表明党参、白术、黄芪、甘草化学提取组分通过调节细胞内ODC活性和多胺生成,对IEC-6细胞增殖、迁移、分化、吸收功能起到促进或抑制作用。提示小肠隐窝细胞是健脾益气中药的主要药理作用靶点。

Achievements of pharmacological studies on IEC-6 small intestinal crypt-like cells (IEC-6 cells) were reviewed. Based on the physiological research of IEC-6 cells, the pharmacological experiment model of IEC-6 cells was established and the pharmacological actions of spleen-strengthening and Qi-invigorating herbal medicine (SQHM) on intestinal epithelial stem cells were observed. Thiazolyl blue colorimetry (MTF), reverse transcription polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), high performance liquid chromatography (HPEC) and immune fluorescence labeling technology were applied in the experiments to observe the effect of SQHM on the proliferation, migration and differentiation of IEC-6. Its influences on the expression of villus protein, divalent mental transferrin, interleukin-6 (IL-6) and ornithine decarboxylase (ODC) and on ODC activity and putrescine content were also observed. The results showed that the extracted chemical components of Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Radix Astragali seu Hedysari and Radix Glycyrrhizae promoted or inhibited the proliferation, migration, differentiation and absorption function of IEC-6 cells by regulating ODC activity and putrescine production. It is indicated that IEC-6 cells are the pharmacological target of spleen-strengthening and Qi-invigorating herbal medicine.