健胎液对米非司酮处理的小鼠子宫雌孕激素受体基因表达的影响

Effect of Jiantai Liquid on Expression of Estrogen and Progesterone Receptors in Uterus of Mice with Embryo Implantation Dysfunction Induced by Mifepristone

目的探讨健胎液改善着床障碍小鼠子宫内膜容受性的分子机制。方法利用米非司酮诱导胚泡着床障碍小鼠模型 ,予健胎液进行干预 (中药组 ) ,于妊娠第 8天处死小鼠 ,采用蛋白印迹 (Westernblot)、逆转录聚合酶链式反应 (semi quantitativereversetranscriptasepolymerasechainreaction ,RT PCR)检测子宫雌、孕激素受体 (estrogenreceptor,ER ;progesteronereceptor,PR)蛋白及其基因表达。 结果中药组ER、PR蛋白及基因表达均较模型组显著提高 (P <0 0 5 ) ,与正常组比较差异无显著性 (P >0 0 5 )。结论健胎液通过调节胚泡着床障碍小鼠子宫组织ER、PR蛋白及基因的表达 ,促进子宫内膜发育 。

Objective To explore the molecular mechanism of Jiantai Liquid (JTL) in improving endometrial receptivity of mice with embryo implantation dysfunction (EID). Methods Mice model of EID induced by mifepristone were intervened with JTL (Twig of Chinese Taxillus, Red Sage root, Chinese Angelica, Milkvetch root, Chuanxiong rhizome), and sacrificed on day 8 of pregnancy. The endometrial estrogen receptor (ER) and progesterone receptor (PR) protein and their gene expressions were assessed by Western blot and semi quantitative reverse transcriptase polymerase chain reaction (RT PCR). Results Levels of ER and PR protein and their gene expressions in the JTL treated group were significantly higher than those in the model group respectively (all P<0 05), and showed insignificant difference from those in the normal control group (all P>0 05). Conclusion JTL could promote the development of endometrium and improve the embryo implantation by way of regulating the levels of ER and PR protein and gene expression in mice with EID.