摘要
目的比较黄芪多糖和小檗碱对脂肪细胞糖代谢和细胞分化的影响 ,并分析其改善糖代谢的可能机制。方法检测黄芪多糖和小檗碱干预的脂肪细胞对3 H 葡萄糖的摄取 ,对黄芪多糖和小檗碱干预分化的细胞进行油红O染色并通过比色定量分析脂肪细胞分化程度。逆转录多聚酶联反应 (RT PCR)检测脂肪细胞分化相关基因过氧化物体增殖剂活化受体γ(PPARγ)、CAAT/增强子结合蛋白α(C/EBPα)mRNA的表达。结果黄芪多糖和小檗碱组葡萄糖摄取率分别为正常组的 1 0 9 3 %和 1 82 7%;黄芪多糖明显促进分化及其PPARγmRNA表达 ,与对照组比较 ,差异有显著性 (P <0 0 1 ) ;小檗碱则明显抑制细胞分化及PPARγ、C/EBPαmRNA表达 ,与对照组比较 ,差异有显著性 (P <0 0 1 )。结论黄芪多糖促进脂肪细胞葡萄糖摄取及细胞分化和PPARγmRNA表达 ,而小檗碱促进葡萄糖摄取但抑制脂肪细胞分化和PPARγ及C/EBPαmRNA的表达。
Objective To compare the effects of Astragalus polysaccharides (AP) and berberine (BB) on the adipocyte's carbohydrate metabolism and cell differentiation, for assessing the possible mechanism of them in improving carbohydrate metabolism. Methods Adipocytes were treated with AP or BB, the 3H glucose up take rate in them was investigated, those of differentiation phase were stained by oil red O to analyze the degree of cell differentiation by spectrophotography quantitatively. The adipocyte differentiation related expression of PPARγ mRNA and C/EBPα mRNA were determined by RT PCR. Results The 3H glucose up take rate in the AP group and BB group were 109.3% and 182.7% of that in the blank control group respectively. AP obviously promoted the cell differentiation and up regulated expression of PPARγ mRNA, while BB suppressed the differentiation and expression of PPARγ and C/EBPα mRNA distinctly, all showing significant difference as compared with that in the blank control (P<0.01). Conclusion AP could promote glucose up take, cell differentiation and PPARγ mRNA expression, BB also promote glucose up take, but suppress the cell differentiation, and inhibit expressions of PPARγ and C/EBPα mRNA in 3T3 L1 adipocytes.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第10期926-928,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
教育部 985工程资助项目 (No .2 0 0 1 - 1 3)