分子吸收光谱在生物大分子研究中的应用

Application of Molecular Absorption Spectrophotometric Method to the Determination of Biologic Macromolecular Structures

综述了近年来分子吸收光谱法在生物大分子结构研究中的应用 ,重点介绍了利用分子吸收光谱法研究蛋白质、核酸结构及其与小分子的相互作用。分子吸收光谱法因其简便、快速的特点而广泛应用于生物大分子结构的测定。紫外光谱法常用于从分子水平上研究小分子如抗癌药物与核酸作用的机理 ,并应用于小分子作为光谱探针测定蛋白质、核酸构象的变化。傅里叶变换红外光谱法主要应用于蛋白质二级结构的测定 ,运用傅里叶自卷积将重叠的酰胺带进行处理 ,获取蛋白质二级结构的含量信息 。

A review on recent applications of molecular absorption spectrophotometric method to the identification of the structures of biologic macromolecules, such as protein and nucleic acid, is presented. Molecular absorption spectrophotometric method is widely used in the structure determination of biologic macromolecules for its convenience and speed. Ultraviolet absorption spectrum (UV) can be (used) in the research on the mechanism of the interaction of anticancer drugs and DNA. UV can also be used to study the interaction of spectroscopic probe with biologic molecule and their binding mechanism. Protein secondary structure and conformation can be (investigated) by Fourier infrared spectroscopy (FTIR) deconvolution analysis. Molecular absorption spectrophotometric method is an (important) tool for structure study of biologic macromolecules.