浮游植物活体三维荧光光谱分类判别方法研究

Research on Discrimination of 3D Fluorescence Spectra of Phytoplanktons

选取中国东海 6种分属于 4个门类的赤潮藻种、优势种在实验室进行扩大培养 ,测量了 2个温度(2 0和 15℃ )、3个光照 (70 0 0 ,4 10 0和 110 0Lux)、不同生长期浮游植物的三维激发 /发射荧光光谱 ,对浮游植物活体三维荧光光谱分类判别方法进行了研究。结果表明 :基于主成分的判别分析法是利用三维光谱数据判别浮游植物种类的有效方法 ,对于金藻is ,绿藻 pl ,中肋骨条藻sk三个物种 ,区分识别率理想 ;可以将中肋骨条藻 (硅藻 )与甲藻al和

Six phytoplankton species belonging to four divisions, which may cause red tide, were selected and cultivated under different temperature(20 degreesC and 15 degreesC) and illumination(7 000, 4 100 and 1 100 Lux) in lab. 3D fluorescence spectra were measured in each growth period. Discrimination of these phytoplanktons' 3D fluorescence spectra was explored using discriminant analyses. The result showed that discriminant analyses based on the principal component analysis of spectra data was an efficient method. Isochrysis galbana, Platymonas helgolanidica and Skeletonema costatuma can be discriminated effectively. Skeletonema costatuma, which is an diatom, can be distinguished from Alexandrium tamarense and Prorocentrum micans which belong to Dinophyta.