急性乐果中毒大鼠实验治疗研究

Experimental treatment of acute Rogor poisoning in rats

目的 探讨不同剂量氯磷定和(或)阿托品治疗对急性乐果中毒大鼠的疗效,为改进急性乐果中毒的治疗提供线索。方法 通过2 LD50乐果灌胃染毒建立急性有机磷中毒(AOPP)模型,并将60只SD大鼠随机分为对照组、未治疗组、阿托品治疗组、小量氯磷定组、中等量氯磷定组、突击量氯磷定组,观察不同治疗情况下中毒大鼠病情变化特点及其规律。结果 单用阿托品治疗可改善乐果中毒大鼠流涎、流泪、肌颤、肌无力、呼吸困难等症状,可挽救大部分大鼠生命,加用氯磷定治疗,可使呼吸困难、肌力下降、全身肌颤等症状较单用阿托品延迟出现,症状减轻,持续时间缩短,呼吸困难发生率、病死率明显下降,阿托品用量减少,且随剂量增加,效果更加明显,以突击量氯磷定效果最佳,染毒大鼠存活率100％。结论 氯磷定联合适量阿托品对急性乐果中毒疗效确切,且以突击量氯磷定效果最佳。

Objective To examine the therapeutic effect of different dose of pralidoxime-Cl(PAM-Cl)and (or)atropine on acute Rogqr poisoning in rats.Methods An experimental model of AOPP was made by giving 2LD50 Rogor to tats itragastrically,according to the therapy,60 SD rats were assigned randomly to the control(A)group,the untreated(B)group,the atropine treated(C)group,the low- dose PAM - Cl treated(D) group, the middle - dose PAM - Cl treated(E) group, the concentrated - dose PAM - Cl treated(F) group. The clinical manifestation of rats in different group were observed. Results Atropine could alleviate the symptoms (such as slaver, tear, myopalmus, myashenia dyspnea, etc) of Rogor poisoned rats and most rats in atropine treated group survived; The main symptoms such as dyspnea, myasthnia,myopalmus of rats in group D, E or F appeared later and lasted shorter time than that in group C, and the main symptoms were slighter. The incidence of dyspnea and the fatality rate were lower in group D, E or F than those in group C, and the dosage of atropine in group D, E or F were fewer than those in group C; The most effective dose of MPA - Cl was the concentrated dose , the livability of rats in group F was 100% .Conclusion The therapeutic effect of combined use of PAM - Cl and suitable dose of atropine in acute Rogor poisoning was significant, and the most effective dose of PAM - Cl was the concentrated dose.