乳腺癌组织中p33^(ING1b)和p^(53)及C-erbB-2表达对预后评估的意义

Expression of p33^(ING1b),p^(53) and C erbB-2 in breast carcinoma tissue in relation to clinical significance of prognosis

目的:探讨p33ING1b,p53,原癌基因C-erbB-2在乳腺癌的发生发展中的生物学特征及其对预后评估的意义。方法:应用EVISION免疫组化法,检测了80例乳腺癌标本中C-erbB-2,p33ING1b,p53的表达,及13例癌旁正常组织p33ING1b的表达。结果:乳腺癌组及对照组中p33ING1b均阳性表达,乳腺癌组织中表达犤65%(52/80)犦有明显下降,两组之间差异有显著性意义(χ2=25.138,P<0.01),p33ING1b,p53,C-erbB-2在乳腺癌组织中表达阳性率分别为65%,60%,61%。其中p33ING1b的表达与雌激素受体和孕激素受体水平呈正相关(r=0.371,r=0.537,P<0.05)p33ING1b,p53与肿瘤大小、腋窝淋巴结有无转移呈负相关(r=-2.09,r=-0.537,P<0.05)。C-erbB-2与组织学分级,淋巴结有无转移呈正相关(r=0.371,P<0.05),与雌、孕激素受体水平呈负相关(r=-0.387,r=-0.440,P<0.05)。结论:p33ING1b基因的表达可能需要p53基因的协同,两者共同作用于细胞的生长、凋亡和转化,保证机体发挥正常的功能,抑制肿瘤的发生。ING1是抑癌基因,其蛋白p33ING1b在乳腺癌中低表达,对乳腺癌的发生发展可能起重要作用,p33ING1b,p53,C-erbB-2可作为判断乳腺癌恶性程度及预后的重要指标。

AIM:To probe into the biological characters of p33ING1b,p53,C erbB 2 in the happening and development of breast carcinoma and its clinical signification. METHODS:The expression of C erbB 2,p33ING1b,p53 of 80 specimens of breast carcinoma and the expression of p33ING1b of 13 juxtacancerous tissue were detected with EVISION immunohistochemical method. RESULTS:The expressions of p33ING1b in breast carcinoma group and control group were positive,65%(52/80) of the expression of breast carcinoma tissue in breast carcinoma group was extremely decreased,significantly different from the other group(χ2=25.138, P< 0.01). The positive rates of p33ING1b,p53,and C erbB 2 expression were 65%,60%,and 61%respectively.The expression of p33ING1b was positively correlation with the level of estrogen receptor(ER) and progestogen receptor(PR)(r=0.371,r=0.537,P< 0.05),and the expression of p33ING1b,p53 were negatively correlation with tumor size and lymph node metastases(r=-2.09,r=-0.537,P< 0.05). The expression of C erbB 2 were positively correlation with histological grading and lymph node metastases(r=0.371,P< 0.05),and they were negatively correlation with the level of ER and PR(r=-0.378,r=-0.440,P< 0.05) CONCLUSION:The expression of p33ING1b gene may be cooperative with p53,and both of them cooperate on the growth,apoptosis and conversion of cells to ensure the normal function of the organism and inhibit the happening of oncology.ING1 is a tumour suppressor gene, its p33ING1b expression in carcinoma of breast is low,and plays an important role in the breast carcinogenesis and development.The p33ING1b,p53,C erbB 2 expression can be used as an important indicator for differentiating malignancy and prognosis of breast carcinoma.