骨延长区成骨方式的实验观察

Expermertal observation of osteoblast mode in bone lengthening area.

目的 了解骨延长区成骨方式的组织学特点 ,为探索加速骨延长区新骨再生与成熟的方法提供组织学依据。方法 3 2只新西兰白兔造成胫骨中段骨延长模型。并随机分成延长 3d、延长 7d、延长结束时、延长结束后 5d、1 0d、1 5d、2 0d、3 0d、5 0d、60d ,共 1 1个亚组。定期在麻醉下取材 ,常规方法制作光镜、电镜切片 ,分别在光、电镜下观察。结果 骨延长区成骨方式有以下 4种 :①发生于骨膜下、延长区中央假性生长板的膜内成骨 ;②发生于骨膜下、假性生长板和软骨岛的软骨内成骨 ;③皮质骨成骨 ;④由成纤维细胞成骨的纤维内成骨。结论 骨延长区骨组织的修复方式与骨折愈合的方式有部分相似之处。由于骨延长区骨组织的修复是在牵张应力的刺激下进行 ,所以其成骨方式呈多样性的修复方式。

Objective To know the histological characteristic of the bone formation mode in bone lengthening,and to provide histological gist for exploring new method to accelerate neoosteogenesis and bone regenerate formation in the distracted area.Methods Thirty-two New Zealand rabbits were selected.Bone lengthening was performed in the middle of the left tibia.All animals were randomly divided into the following subgroups:elongating-3-day group,7-day group,end elongating group,after elongating 5-day group,10-day group,15-day group,20-day group,30-day group,50-day group,60-day group.In the scheduled time the interesting bone tissue was got under anaesthesia.Slices wese made and observed under electron microscopy and optical microscopy.Results There were four types of bone formation mode in bone lengthening area:1.Membranous ossification,which appeared under periosteum and in the pseudo-growth plate;2.Enchondral ossification,which appeared undder periosteum,in the pseudo-growth plate or in cartilaginous nodules;3.Cortical osteogenesis;4.Neoosteogenesis in the distration zone proceeds directly from fibroblast cells.Conclusion There are some resemblance between fracture healing and bone repairing in bone lengthening.For the bone repairing in bone lengthening is under the distact force,its formation mode has its own characteristics.It is multiform.Especially,Neoosteogenesis form fibroblast is most important in bone lengthening.