T细胞疫苗抑制自身免疫性甲状腺炎的发生

T cell vaccination prevents experimental autoimmune thyroiditis

目的 研究T细胞疫苗 (TCV)在小鼠实验性自身免疫性甲状腺炎 (EAT)发生中的阻断作用及可能机制。方法 磁珠分离CD4 + T细胞 ,体外活化 ,戊二醛处理获得T细胞疫苗 ,体内接种EAT小鼠。通过EAT评价、细胞因子测定及细胞增殖试验了解TCV对EAT的阻断作用 ;流式细胞术测定小鼠CD4 + CD2 5 + T细胞百分率。结果 TCV接种后小鼠体内自身抗体水平明显下降 ,甲状腺无明显病理变化 ,IL 2和IFN γ水平以及Tg刺激的淋巴细胞增殖能力均显著降低 ,同时CD4 + CD2 5 + T细胞百分率较EAT组有明显增高。结论 TCV接种能明显抑制小鼠EAT的发生 ,可能与机体内CD4 + CD2 5 +

Objective To investigate the prevention of mouse experimental autoimmune thyroiditis (EAT) induced by T cell vaccination (TCV). Methods Mouse CD4 + T cells were isolated by magnetic activated cell sorting (MACS), and activated with dendritic cells and thyroglobulin, and then expanded in the condition medium containing ConA and IL-2 in vitro. These cells were collected and treated with glu- taraldehyde as TCV. The inhibiting effect of TCV on EAT was detected by autoantibody and cytokines assay, pathological assessment, and lymphocytes proliferation test. The percentage of CD4 +CD25 +T cell was analyzed by Flow cytometry (FCM). Results The levels of thyroglobulin autoantibody markedly decreased and there was no significant change in thyroid after administration of TCV. The proliferation of lymphocytes, stimulated by thyroglobulin, IL-2, and IFN-γ, was decreased, but the percentage of CD4 +CD25 +T was increased. Conclusion The data suggests that TCV can effectively prevent EAT, which may be used as a novel therapeutic strategy.