感染大链壶菌后蚊幼虫脂肪、酯酶和脂肪酶的变化

Change of Lipid,Esterase and Lipase in Mosquito Larvae Infected with Lagenidium giganteum

目的 从蚊虫生理生化代谢角度探讨大链壶菌 (Lagenidiumgiganteum )灭蚊机制。 方法 采用组织化学方法检测正常致倦库蚊 (Culexquinquefasciatus)和白纹伊蚊 (Aedesalbopicus)幼虫于感染大链壶菌后不同时间两种蚊幼虫体内的脂肪含量、酯酶和脂肪酶活性 ,进行显微摄影和定量图像分析。 结果 感染后 2 4h ,致倦库蚊幼虫体内的脂肪含量低于对照组 ,而酯酶和脂肪酶活性高于对照组 ;感染后 48h和 72h ,两种蚊幼虫体内的脂肪含量均明显低于对照组 ,酯酶和脂肪酶活性明显高于对照组。 结论 大链壶菌感染引起蚊幼虫体内脂肪含量减少 ,而与脂肪水解相关的酯酶和脂肪酶活性升高 ,导致体内脂肪代谢紊乱 。

Objective To explore possible physiological and biochemical mechanisms of Lagenidium giganteum infection in killing mosquito larvae. Methods The content of lipid and the activities of esterase and lipase between the normal and infected mosquito larvae were observed with histochemical method. The results were photomicrographed and analysed by image analysis using computer. Results In 24 hrs after infection, the content of lipid in the infected Culex quinquefasciatus larvae was lower while the activities of esterase and lipase in the larvae were higher than the control. In 48 hrs and 78 hrs after infection, the content of lipid in the infected larvae of C. quinquefasciatus and Ae. albopictus were significantly lower while the activities of esterase and lipase were significantly higher than those of control. Conclusion The content of lipid in the mosquito larvae decreased while the activities of esterase and lipase increased after the infection of Lagenidium giganteum . The disorder of lipid metabolism might be part of the killing mechanisms to C. quinquefasciatus and Ae. albopictus larvae.