明胶酶A在甲状腺乳头状癌中的表达及临床意义

Expression of selatinase in papillary thyroid carcinoma and its clinical significance

目的 :探讨明胶酶A在甲状腺乳头状癌中的表达及其与甲状腺乳头状癌侵袭与转移的关系 ,方法 :用原位杂交、免疫组化SP法检测95例甲状腺乳头状癌及 5 9例癌旁组织中明胶酶AmRNA、蛋白的表达水平。结果 :明胶酶AmRNA和蛋白在甲状腺乳头状癌中的阳性率分别为 74 7% ( 71/95 )、77 9% ( 74/95 ) ,而在癌旁组织中的阳性率分别为 10 2 % ( 6/5 9)、2 5 4% ( 15 /5 9) ,两者差异有统计学意义 ,P <0 0 1。明胶酶AmRNA及其蛋白在甲状腺乳头状癌 (微小癌、腺内型、腺外型 )的阳性率分别为5 9 3 % ( 16/2 7)、 80 0 % ( 4 0 /5 0 )、 83 3 %( 15 /18) ;66 7% ( 18/2 7)、 80 0 % ( 4 0 /5 0 )、88 9% ( 16/18) ,癌组织各型间差异无统计学意义 ,P >0 0 5。淋巴结有癌转移组的阳性率分别为 91 4% ( 3 2 /3 5 )、97 1% ( 3 4/3 5 ) ,淋巴结无癌转移组的阳性率分别为 65 0 % ( 3 9/60 )、66 7%( 4 0 /60 ) ,两者差异有统计学意义 ,P <0 0 1。结论 :明胶酶A在甲状腺乳头状癌中呈高表达 。

OBJECTIVE: To study the expression of selatinase mRNA and protein in PTC and its clinical significance.METHODS:In situ hybridization and immunohistochemistry(SP method)were used to detect selatinase mRNA and protein.RESULTS:The positive expression rates of selatinase mRNA and protein in PTC were 74.7%(71/95) and 77.9%( 74/95) respectively, but in nearby no-tumorous tissues,only 10.2%(6/59) and 25.4%(15/59),and there was a significant difference,P<0.01.Moreover, the positive expression rates of selatinase mRNA and protein were 59.3% (16/27) and 66.7%(18/27)in papillary microcarcinoma, 80.0%(40/50)and 80.0%(40/50) in intrathyroid carcinoma, 83.3%(15/18) and 88.9%(16/18) in extrathroid carcinoma, respectively.There was no significant difference,P>0.05. The positive rates of Selatinase mRNA and protein with lymph node metastasis were 91.4% (32/35)and 97.1%(34/35),and 65.0%(39/60) and 66.7%(40/60),in patients without lymph node metastasis,P<0.01. CONCLUSIONS:Selatinase mRNA and protein express in PTC highly and are associated with the tumor invasion and metastasis.