金喜素诱导人肝癌HCC7721细胞凋亡及细胞周期阻滞的研究

Topotecan induces apoptosis and cell cycles arrest in human liver cancer HCC7721 cells

目的 :研究金喜素诱导人肝癌HCC772 1细胞的凋亡作用。方法 :采用MTT法测定金喜素对人肝癌HCC772 1细胞的杀伤作用 ;通过电镜观察细胞形态变化 ;琼脂糖凝胶电泳检测断裂DNA ;流式细胞仪分析DNA含量及细胞周期。结果 :经0 3 μmol/L金喜素处理 6、12、2 4及 3 6h ,HCC772 1细胞的存活率与对照组相比 ,逐渐降至 ( 83± 16) %、( 69± 10 ) %、( 5 2±13 ) %和 ( 3 0± 11) % ,低于对照组 ,P <0 0 5 ;靶细胞出现细胞固缩、染色质凝聚和边集 ;产生梯状DNA并检测到亚二倍体峰(凋亡峰 ) ;同时 ,G0 /G1期细胞增多 ,S期细胞减少。结论 :金喜素可抑制人肝癌HCC772 1细胞增殖 ,使细胞阻滞在G0 /G1期 ,进一步诱导细胞凋亡。

OBJECTIVE:To study the proapoptotic effects of topotecan (TPT) on human liver cancer HCC7721 cells.METHODS:The cytotoxic effect of topotecan on HCC7721 cells was measured by MTT assay, the morphological changes observed by a eletronic microscope, DNA fragmentation detected by agarose gel electrophoresis, and the cell cycles and DNA content detected by flow cytometry.RESULTS:After incubated with 0.3 μmol/L topotecan for 6 h, 12 h, 24 h and 36 h, the survival rates in HCC7721 cells significantly reduced to (83±16)%,(69±10)%,(52±13)% and (30±11)%,P<0.05,respectively Targeted cells demonstrated cell shrinkage, chromatin condensation and margination. Ladder DNA and aneuploid peak were detected. Meanwhile, treated by topotecan for 24 h, there was a remarkable accumulation of cells in the G 0/G 1 phase of the cell cycles, and the cells in S phase declined.CONCLUSION:Topotecan can inhibit proliferation in human liver cancer HCC7721 cells,with G 0/G 1 arrest and induction of apoptosis.