摘要
目的探讨白藜芦醇对K562细胞的凋亡诱导效应和对K562细胞内活性氧水平的影响。方法应用噻唑蓝(MTT)比色法、光镜、DNA琼脂糖凝胶电泳和流式细胞术(FCM)检测细胞凋亡;FCM测定细胞内活性氧(ROS)水平。结果白藜芦醇显著抑制K562细胞增殖,并呈现典型的凋亡形态学改变;DNA电泳可见梯状条带出现;FCM分析显示S期细胞数明显增多,出现SG2期阻滞,ROS水平升高。结论白藜芦醇诱导K562细胞凋亡与细胞内活性氧水平升高有关。
AIM:To investigate the apoptosis of K562 cells induced by resveratrol and the influence of intracellular reactive oxygen species (ROS) level. METHODS: MTT assay, light microscopy, DNA agarose gel electrophoresis and cell cycle analysis were used for examine apoptosis in K562 cells. Intracellular ROS level was measured with flow cytometry (FCM). RESULTS: Resveratrol inhibited growth of K562 cells. Agarose gel electrophoresis showed evident DNA fragmentation. Cell cycle analysis indicated increased S phase proportion, as well as apparent S/G 2 phase arrest. After application of resveratrol, the level of the markedly increased of ROS. CONCLUSION: Resveratrol induces apoptosis in K562 cells may be through stimulating ROS production.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第10期1147-1149,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
白藜芦醇
白血病
K562
细胞凋亡
活性氧
resveratrol
leukemia
K562
apoptosis
reactive oxygen species (ROS)
