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人p100蛋白:可增强STAT6基因转录活性的新因子 被引量：6

Human p100 protein:a novel coactivator that enhances ST AT6-mediated gene transcriptional activation
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摘要 目的 :建立可稳定表达人类p10 0蛋白的细胞株 ,研究细胞内p10 0蛋白参与STAT6介导的基因转录调控的机制。方法 :利用免疫共沉淀法检测蛋白与蛋白间结合 ,荧光素酶法测定STAT6基因转录活性。结果 :p10 0既可与STAT6结合 ,亦可与RNA多聚酶Ⅱ结合 ,并能增强STAT6介导的基因转录活性。结论 :p10 0蛋白是STAT6共激活因子 ,可桥连STAT6和基本转录调控元件 ,促进STAT6介导的基因转录活性。 Objective:To setup a stable cell line which can overexpress human p100 protein,and then st udy the physiologic functions of p100 in regulation of STAT6-mediated gene tran scription.Methods:Using coimmunoprecipitation method to identify protein-protein interactions.STA T6-mediated gene transcriptional activation was detected by luciferase assay.Results:Human p100 protein interacted with both STAT6 and RNA polymerase Ⅱ,resulting in the enhancement of STAT6-mediated gene transcriptional activation.Conclusion:Human p100 protein acts as a bridging factor between STAT6 and basal transcripti onal machenary. [
作者 杨洁 姚智 郁春艳 Olli Silvennoinen
机构地区 天津医科大学免疫学教研室 芬兰坦佩雷大学医学技术研究所
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2004年第11期739-742,共4页 Chinese Journal of Immunology
基金 国家自然科学基金资助项目 (批准号 :3 0 3 0 0 0 70 )
关键词 STAT6 人类p100蛋白 RNA多聚酶Ⅱ 基因转录 STAT6 Human p100 protein RNA polymerase Ⅱ Gene transcription
分类号 R392.11 [医药卫生—免疫学]
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参考文献7

  • 1[1]Quelle F W, Shimoda K,Thierfelder W et al. Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis [J].Mol Cell Biol,1995; 15: 3336-3343.
  • 2[2]Muller M, Briscoe J, Laxton C et al. The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction [J]. Nature, 1993;366:114-116.
  • 3[3]Wei D, Le X, Zheng L et al. Stat3 activation regulates the expression of vascular endothelial growth factor and human pancreatic cancer angiogenesis and metastasis [J]. Oncogene,2003;22(3) :319-329.
  • 4[4]Duetsch G, Illig T, Loesgen S et al. STAT6 as an asthma candidate gene:polymorphism-screening,association and haplotype analysis in a Caucasian sib-pair study [J]. Hum Mol Genet, 2002; 11 (6): 613-621.
  • 5[5]Blease K, Schuh J M, Jakubzick C et al. Stat6-deficient mice develop airway hyperresponsiveness and peribronchial fibrosis during chronic fungal asthma [J]. Am J Pathol,2002; 160(2) :481-490.
  • 6[6]Shuai K. Modulation of STAT signaling by STAT-interacting proteins [J].Oncogene,2000; 19:2638-2644.
  • 7[7]Tong X, Drapkin R,Yalamanchili R et al. (1995) The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE [J].Mol Cell Biol,1995;15:4735-4744.

同被引文献70

  • 1[1]MCPHEETERS D S,MUHLENKAMP P.Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast[J].Mol Cell Biol,2003,23:4174-4186.
  • 2[2]TURNER IA,NORMAN CM,CHURCHER MJ,et al,Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome[J].RNA,2006,12(3):375-386.
  • 3[3]JEREMY R S,JAVIER F C.Pre-mRNA splicing:life at the centre of the centraI dogma[J].J Cell Science,2004,117:6261-6263.
  • 4[4]LUO H R,MOREAU G A,LEVIN N,et al.The human Prp8 protein is a component of both U2-and U12-dependent spliceosomes[J].RNA,1999,5:893-908.
  • 5[5]TAKAHASHI A,MURAMATSU H,TAKAGI S,et al.A splicing factor,Prp8:Preferential localization in the testis and ovary in adult mice[J].Biochem,2001,129:599-606.
  • 6[6]BOON K L,NORMAN C M,GRAINGER J,et al.Prp8p dissection reveals domain structure and protein interaction sites[J].RNA,2006,12(2):198-205.
  • 7[7]KIELKOPF C L,LUCKE S,GREEN M R.U2AF homology motifs:Protein recognition in the RRM world[J].Genes Dev,2004,18:1513-1526.
  • 8[8]BELLARE P,KUTACH AK,RINES AK,et al.Ubiquitin binding by a variant Jabl/MPN domain in the essential pre-mRNA splicing factor Prp8p[J].RNA.2006,12(2):292-302.
  • 9[9]COLLINS C A,GuTHRIE C.Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome[J].Genes Dev,1999,13:1970-1982.
  • 10[10]GRAINGER R J,BEGGS J D.Prp8 protein:At the heart of the spliceosome[J].RNA,2005,11:533-557.

引证文献6

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中国免疫学杂志
2004年 第11期

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