4种中药橡胶膏剂中樟脑、薄荷脑、冰片和水杨酸甲酯含量的气相色谱法测定

Determination of camphor, menthol, synthetic borneol and methyl salicylate in four kinds of TCM adhesive plasters by GC

目的 :用气相色谱法同时测定伤湿止痛膏、安阳精制膏、少林风湿跌打膏和风湿止痛膏中樟脑、薄荷脑、冰片和水杨酸甲酯的含量。方法 :以萘为内标物 ,采用内标物预先加入法 ,用挥发油测定器蒸馏制备供试液。以 1 0 %的PEG 2 0M为固定相 ,玻璃填充柱 ,氮气为载气 ,柱温 1 30℃ ,FID检测器。结果 :在该色谱条件下 ,4种制剂样品中的樟脑、薄荷脑、冰片 (异龙脑和龙脑 )、水杨酸甲酯及内标物萘均得到良好的分离。用风湿止痛膏进行方法学研究 ,樟脑、薄荷脑、冰片和水杨酸甲酯的加样回收率依次为 95 .5 4 %(RSD =1 .6 %) ,96 .90 %(RSD =2 .2 %) ,97.30 %(RSD =1 .4 %) ,和 96 .4 8%(RSD =2 .8%)。用所建立的方法对 4种中药橡胶膏剂的 6批样品进行含量测定 ,均取得满意的结果。结论 :本法灵敏、准确、重现性好 ,可用于控制这类制剂的质量。

AIM: To establish the determination for camphor, menthol, synthetic borneol, and methyl salicylate in four kinds of TCM adhesive plasters by GC. METHODS: The internal standard method was employed. Naphthalene, the internal standard, was added to the sample before treatment. The sample solution was prepared by distillation with essential oil collector. The GC system consisted of glass column, 10% PEG 20M as the stationary phase, nitrogen as the carrier gas, column temperature at 130℃, and FID as the detector. RESULTS: Camphor, menthol, synthetic borneol(borneol and isoborneol), and methyl salicylate in 6 lots of samples and naphthalene have been separated well under the chromatographic condition. In the methodology study on Fengshi Zhitong Adhesive Plasters, the average recovery of camphor, menthol, synthetic borneol, and methyl salicylate were 95.54%( RSD =1.6%), 96.90%( RSD =2.2%), 97.30%( RSD =1.4%), and 96.48%( RSD =2.8%), respectively. CONCLUSION: The method is sensitive, accurate and reproducible, and can be used to control the quality of the four kinds of preparations.