RP-HPLC测定酸枣仁汤中芒果苷和甘草酸的含量

Determination of mangiferin and glycyrrhizic acid in Suanzaoren Decoction by RP-HPLC

目的 :建立测定酸枣仁汤 (炒酸枣仁、茯苓、川芎、知母、甘草 )中芒果苷和甘草酸含量的反相高效液相色谱分析方法。方法 :色谱柱 :Hypersil C1 8(5 μm ,2 0 0mm× 4 .6mmI.D .) ,芒果苷流动相为乙腈 1 %冰醋酸水溶液 (1 3∶87,v/v) ,检测波长 32 0nm ,内标物香兰素 ;甘草酸流动相为甲醇 乙腈 1 %冰醋酸水溶液 (2 5∶1 5∶6 0 ,v/v) ,检测波长 :32 0nm ,内标物甲苯咪唑。流速均为 0 .8mL·min-1 。结果 :芒果苷和甘草酸分别在 1 0 .72～ 85 .76 μg·mL-1 (r =0 .9998)和 0 .0 80～ 0 .80mg·mL-1 (r =0 .9997)范围内线性关系良好。平均回收率分别为 97.2 %和 97.6 %。RSD分别为 3.0 %和 2 .4 %。结论 :本法操作简便 ,快速、准确 ,可用于酸枣仁汤中芒果苷和甘草酸的含量测定。

AIM: To develop an RP HPLC method for determination of mangiferin and glycyrrhizic acid in Suanzaoren Decoction(Semen Ziziphi Spinosae, Poria, Rhizoma Ligustici Chuanxiong, Rhizoma Anemarrhenae, Radix Glycyrrhizae). METHODS: Hypersil C 18 column was used. The mobile phase consisted of acetonitrile water(13∶87,v/v) with 1% glacial acetic acid for mangiferin and methanol acetonitrile water(25∶15∶60,v/v) with 1% acetic acid for glycyrrhizic acid, respectively. Detection wavelengths were set at 320nm and 254nm, respectively. The internal standards were vanillin for mangiferin and mebendazole for glycyrrhizic acid, respectively. The flow rates were both 0.8mL·min -1 . RESULTS: The linear ranges for mangiferin and glycyrrhizic acid were 10.72～85.76μg·mL -1 ( r =0.9998) and 0.080～0.80mg·mL -1 ( r =0.9997), respectively. The average recoveries were 97.2% with RSD 3.0% and 97.6% with RSD 2.4%, respectively. CONCLUSION: This method is simple, rapid and accurate, and can be used for the determination of mangiferin and glycyrrhizic acid in Suanzaoren decoction.