活血健骨胶囊的质量标准研究

Quality standard of Huoxuejiangu Capsules

目的 :拟建立活血健骨胶囊 (丹参、赤芍、黄芪、延胡索、补骨脂等 )的质控方法。方法 :采用薄层色谱鉴别法对活血健骨胶囊中的黄芪、延胡索、赤芍和补骨脂定性 ,HPLC法测定的丹参酮ⅡA 含量。结果 :在薄层色谱中能检出黄芪、赤芍、延胡索和补骨脂 ;丹参酮ⅡA 在 0 .1 0 6～ 2 .1 2 μg范围内呈线性关系 ,r =0 .9996 ,平均回收率为 98.6 %,RSD为1 .4 8%。结论 :所建立的方法简便可行 ,重现性好 ,为活血健骨胶囊质量控制提供了方法。

AIM: To establish the quality standard of Huoxuejiangu Capsules (Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Radix Astragali seu Hedysari, Rhizoma Corydalis, Fructus Psoraleae, etc.). METHODS: Radix Astragali, Rhizoma Corydalis, Radix Paeoniae Rubra, Fructus Psoraleae were identified by TLC. The content of tanshinone Ⅱ A was determined by HPLC. RESULTS: Radix Astragali, Rhizoma Corydalis, Radix Paeoniae Rubra, Fructus Psoraleae could be dtected by TLC. Tanshinone Ⅱ A showed a good linear relationship at the range of 0.106～2.12μg, r =0.9996. The average recovery was 98.6%, and RSD was 1.48%. CONCLUSION: The method established is simple, feasible and reproducible. This study provided a method for quality control of Huoxuejiangu Capsules.