小檗碱对大鼠胃窦平滑肌细胞收缩作用的影响(英文)

Contractile effect of berberine on isolated antral smooth muscle cells in rat stomach

目的 :观察小檗碱对大鼠胃窦平滑肌细胞的收缩作用。方法 :采用Bitar法分离制备大鼠胃窦平滑肌细胞悬液 ;将 3种不同浓度的小檗碱加入细胞悬液中 ,用测微尺观察细胞长度的变化。结果 :与对照组相比 ,终浓度为 0 .5 g·L-1 ,1 g·L-1 的小檗碱可显著促进大鼠胃窦平滑肌细胞收缩 (P <0 .0 5 ) ,并呈剂量 效应关系。结论 :小檗碱对大鼠胃窦平滑肌细胞有直接收缩作用。

AIM: To investigate the contractile effect of berberine on antral smooth muscle cells in rat stomach. METHODS: Smooth muscle cells were isolated from the antrum of the SD rat stomach. Different doses of berberine were added into cell suspension. Contractibility of smooth muscle cells were measured by micrometry. RESULTS: Compared with the control group the length of the treated cells(0.5g·L -1 ,1g·L -1 ) was significantly short( P <0.05) and the correlation of Dose Response was observed. CONCLUSION: Berberine has a direct contractile effect on the antral smooth muscle cells in rat stomach.