中国南海海绵正丁醇部分中的核苷类成分研究

Study on nucleotidoids from n-butyl alcohol part of sponge Cinachyrella australiensis

目的 对中国南海产海绵 Cinachyrella australiensis的正丁醇部分化学成分进行了系统分离 ,为合理开发海洋药物资源 ,更好地利用海洋生物体内具有强生理活性的化学成分 ,从而开发出适用于人体的安全有效的药物。方法 采用多种色谱方法进行分离纯化 ,应用波谱分析技术 ,并结合文献对照 ,对所分离到的化合物进行了结构鉴定。结果 共分离得到 7个化合物 ,其结构分别为 :2 -甲基 - 6 -氨基嘌呤脱氧核苷 [2 - m ethyl- 6 - am ino- 9- (2 - deoxy- β-D- ribofuranosyl) - purine, ]、6 -氨基嘌呤脱氧核苷 (2′- deoxyadenosne, )、6 -氨基嘌呤核苷 (6 - am ino- 9- β- D- ribo-furanosyl- 9H- punne, )、尿嘧啶 (uracill, )、胸腺嘧啶 (thym ine, )、胸腺嘧啶脱氧核苷 (thymidine, )、尿嘧啶脱氧核苷 [1- (2 - deoxy- β- D- ribofuranosyl) uracil, ]。结论 对中国南海海绵正丁醇部分的化学成分进行了报道 ,系统地分离出了 2大类核苷类化合物。

Object To search for bioactive secondary metabolites from marine organisms, the marine sponge Cinachyrella australiensis was collected from Hainan Island, Southern China Sea to develop the efficient drug for human beings. Methods Silic gel flash chromatography in association with reverse phase semipreparative HPLC was performed for the isolation and purification of the ethanol extracts, and extensive spectroscopy including 2D NMR spectra as well as the comparison of the spectral data with those reported in literatures was applied for the structure elucidation. Results Seven nucleotidoids were isolated and identified. They are named as 2-methyl-6-amino-9-(2-deoxy-β-D-ribofuranosyl)-purine (Ⅰ), 2′-deoxyadenosne (Ⅱ), 6-amino-9-β-D-ribofuranosyl-9H-punne (Ⅲ), uracill (Ⅳ), thymine (Ⅴ), thymidine (Ⅵ), 1-(2-deoxy-β-D-ribofuranosyl) uracil (Ⅶ). Conclusion The sponge C. australiensis is systemically separated, and then two kinds of nucleotidoids compound are extracted.