H_2O_2-Luminol流动注射-抑制化学发光法测定银杏黄酮

Determination of flavonoids of Ginkgo biloba by H_2O_2-Luminol chemiluminescence inhibition with flow injection

基于在碱性条件下,银杏黄酮对H2O2 Luminol体系有显著的抑制作用,结合反相流动注射技术,首次建立了流动注射 抑制化学发光测定银杏提取物中的银杏黄酮。银杏黄酮质量浓度在0.2～20.0μg mL范围与相对发光强度呈线性关系,检出限为0.1μg mL,采样频率为140次 h,对10.0μg mL的银杏黄酮平行测定11次,其RSD为1.5%,回收率为100.0%～100.2%。该方法可用于银杏黄酮制剂的测定。

A reverse flow injection chemiluminescence inhibition method for the determination of flavonoids of Ginkgo biloba was firstly described. It was found that the CL intensity of the H_2O_2-luminol system could be inhibited in the presence of flavonoids of Ginkgo biloba. The decrease of CL emission was correlated with flavonoids of Ginkgo biloba concentration in the range from 0.2 to 20.0 μg/mL. The detection limit was 0.1 μg/mL and the relative standard deviation was 1.5%(c=10.0 μg/mL,n=11). A sampling frequency of 140/h was attained. The method was applied to the determination of flavonoids of Ginkgo biloba with recoveries of 100.0% and 100.2%.