高效液相色谱法测定通便灵胶囊中大黄酸的含量

Quantitative determination of Rhein in Tongbianling capsule by HPLC

目的 :建立 HPL C法测定通便灵胶囊中大黄酸含量的方法。方法 :采用 Shim- pack VP- ODS(15 0 mm× 4 .6mm,5 μm)色谱柱 ;流动相为甲醇 -水 (70∶ 30 ,磷酸调至 p H=3) ;柱温 :2 4 o C;流速 :1.0 ml/ min;检测波长 :4 37nm。结果 :大黄酸线性范围为 0 .132～ 2 .6 4 μg,r=0 .9991,平均回收率 =10 0 .34% ,RSD=1.2 9%。结论 :此方法可做为通便灵胶囊中大黄酸含量测定的方法 ,为制定该制剂的质量控制标准提供了依据。

bject: To develop an HPLC method for quantitative determination of Rhein in Tongbianling capsule. Methods: The separation was performed on a Shim-pack VP-ODS column and methanol-water (70∶30) was used as mobile phase, adjusted pH to 3 with Phosphoric acid. The temperature of column was 24 oC, the flow rate was 1.0 ml/min and the UV detection wavelength was 437 nm. Results: The linearity of Rhein was good (r=0.999 1) in the range of 0.132~2.64 μg , the average recovery was 100.34% with RSD 1.29%. Conclusion: This method can be used for the quality control of the capsule as reference.