旋光法测定硫酸阿米卡星注射液含量

Determination of the content of amikacin sulfate injection by polarimetric analysis

目的 :建立硫酸阿米卡星注射液含量测定方法。方法 :以水为溶剂 ,运用旋光法测定硫酸阿米卡星注射液含量。结果 :阿米卡星在 1～ 2 0 mg/ml范围内浓度与旋光度呈良好线性关系 ,回归方程 :C =7.892 7A - 0 .0 35 5 ,r =0 .9999,平均回收率 99.8% ,RSD为 0 .89% (n =5 )。结论 :本法简便易行 ,结果准确 。

Object: To establish a method for determining the content of amikacin sulfate injection. Methods: Polarimetric analysis was used and water as solvent. Results: The linear range of amikacin was 1~20 mg/ml. The regression equation was: C=7.892 7 A-0.035 5(r=0.999 9). The average recovery was 99.8% with the RSD of 0.89 %. Conclusion: The method was convenient, accurate and reliable. It was therefore suitable for the quality control of amikacin sulfate injection.