不同基因型甘蔗愈伤组织培养研究

Studies on the Callus Culture of Different Sugarcane Genotypes

以果蔗拔地拉、广东黄皮果蔗及糖蔗闽糖92-649、ROC16、ROC25为试验材料,研究不同预处理、蔗糖浓度及不同激素水平对甘蔗愈伤组织的诱导、分化的影响。结果表明以PVP水溶液浸泡外植体,可使平均褐化率由43.1％减低到22.4％,愈伤组织诱导率由61.04％提高到71.25％;添加50 g/L的蔗糖浓度可促进愈伤组织的诱导及分化。

The effects of different dosage of sucrose and hormone on the tissue culture of 5 sugarcane genotypes were studied. The results showed that ratios of explant browning were decreased from 43.1% to 22.4%, and the ratios of callus inducing were raised from 61.04% to 71.25%. After the explants soaked in polyvinylpyrrolidone (PVP), the mass-concentration of 50 g/L sucrose was suitable for callus inducing and callus plantlets.