苏州地区肺炎链球菌TEM基因分子流行病学研究

Study on the molecular epidemiology of β-lactamase TEM gene in isolated Streptococcus pneumoniae

目的 探讨肺炎链球菌(Sp)是否存在TEM基因及其流行状况。方法 对2002年9月至2003年4月苏州大学附属儿童医院就诊呼吸道感染患儿痰标本中分离到的23株Sp进行β-内酰胺酶TEM全长基因聚合酶链反应检测及扩增产物序列与美国GenBank已登录的TEM基因序列比较分析。结果 23株Sp中21株检出TEM基因(阳性率91.3％)。有1株(株号SR017,青霉素耐药)TEM基因序列与已登录的均不相同,存在编码子第182位ATG[M]→ATA[Ⅰ]有义突变,为TEM基因家族新成员,并成功以TEM-129型登录GenBank(登录号:AY452662);其余20株TEM基因均为TEM-1亚型,1号株(株号SR001)TEM-1型序列也以首次发现于Sp登录GenBank(登录号:AY392531)。结论 从Sp中检出β-内酰胺酶TEM基因,其携带率达91.3％,其基因型有TEM-129和TEM-1型。这一发现丰富了对Sp青霉素耐药的认识。

Objective To investigate the β-lactamase TEM gene of isolated Streptococcus pneumoniae(Sp) in Suzhou area. Methods Twenty-three strains of Sp were collected from respiratory tract secretions of children with respiratòry diseases in Nov 2002 to Apr 2003 at Children's Hospital of Suzhou University(reference strain ATCCA9619) to build TEM polymerase chain reaction(PCR) system (reference strain E. coli. 9-j53R1 with TEM gene)TEM gene of 23 strains was detected to comparo the sequences with published TEM gene sequences in GenBank for analyzing TEM gene model. Results Twenty-one strains had TEM gene with a positive rate of 91.3%(21/23). TEM-129 gene were confirmed from No. 17(SR017, penicillin resistance) TEM sequence. New discovered TEM-129 sequence had a modification (ATG[M]→ATA[I]) at No. 182 code and published(GenBank: www. ncbi. nlm. nih. gov/nucleotide, AY452662). TEM-1 genes were confirmed from other TEM sequences. New discovered TEM-1 gene of isolated Sp had been published(GenBank: www. ncbi. nlm. nih. gov/nucleotide, AY392531)too. Conclusion Isolated Sp had TEM gene(TEM-129,EM-1 genotype)with a positive rate of 91.3%. The result enriched the understanding of isolated Sp with penicillin resistance.