趋化因子受体CCR5、CCR3在1型糖尿病患者中的表达

Expression of chemokine receptor CCR5 and CCR3 in patients with type 1 diabetes mellitus

目的 研究Th1和Th2 细胞表面特征性的趋化因子受体CCR5、CCR3在 1型糖尿病发病机制中的作用。方法 对 15例新诊断的 1型糖尿病患者、10例 2型糖尿病患者以及 10例非糖尿病患者分离外周血单个核细胞 (PBMC)在体外培养 ,用流式细胞仪检测CD+4CCR5 +及CD+4CCR3 +淋巴细胞 ,并用酶联免疫吸附技术检测细胞培养上清中细胞因子IL 4、IFN γ的水平。结果 1型糖尿病患者外周血中CD+4CCR5 +淋巴细胞数显著高于 2型糖尿病患者和对照组 ,CD+4CCR3 +显著低于其它两组 (均P <0 .0 5 )。 1型糖尿病患者和 2型糖尿病患者PBMC细胞上清液中的IFN γ水平高于对照组 (均P <0 .0 5 ) ,1型糖尿病患者IL 4水平低于其它两组 (P <0 .0 1)。结论 检测PBMC的CCR5、CCR3表达可以作为反映人类 1型糖尿病免疫活动的标志 ,从而为

Objective To investigate the role of chemokine receptor CCR5 and CCR3, characteristic surface markers of Th 1 and Th 2 respectively, in the pathogenesis of type 1 diabetes mellitus (DM). Methods 15 cases of newly diagnosed type 1 diabetics, 10 cases of type 2 diabetics and 10 nondiabetic subjects were studied. Peripheral blood mononuclear cells (PBMC) were isolated from heparinized venous blood by centrifugation over Ficoll-Hypaque. After being cultured (37℃, 5%CO 2, 48 h), cells were stained with fluorescent-labelled-CD + 4CCR5 + and CD + 4CCR3 + antibodies. Three-color flowcytometry analysis was performed on a FACSort flowcytometer. The supernatant of culture cells was collected and IFN-γ and IL-4 were measured by ELISA. Results In type 1 DM, the number of CD + 4+CCR5 + lymphocytes was significantly greater than those in type 2 DM and control group while the expression of CD + 4CCR3 + was lower than the other two groups (all P<0.05). The level of IFN-γ was higher in type 1 DM and type 2 DM than that in control group (both P<0.05). The level of IL-4 in thepatientswithtype1diabetes was lower than those in the other two groups (both P<0.05). Conclusion Expressions of CCR5 and CCR3 reflect the immune activity in type 1 DM and may serve as markers for screening type 1 diabetes in early stage.