IgE高亲和力受体β链基因与中国湖北成人变应性哮喘的关系

Association analysis of FcεRⅠβ gene with allergic asthma in Chinese

目的 :探讨IgE高亲和力受体 β链基因 (FcεRⅠβ)启动子 - 10 9位和编码区Glu2 37Gly基因多态性与湖北成人变应性哮喘及血浆总IgE的关系。方法 :采用聚合酶链反应 -限制性片段长度多态性技术检测FcεRⅠβ基因启动子区 - 10 9位和编码区Glu2 37Gly两位点多态性 ,采用病例 -对照法共研究 10 6例成人变应性哮喘患者和 10 6例相匹配的对照者。结果 :①成人变应性哮喘患者FcεRⅠβ基因启动子区 - 10 9位T/T、T/C和C/C基因型频率是0 4 15、0 4 91和 0 0 94 ;与对照相比差异无显著 (χ2 =0 5 5 75 ,P >0 0 5 ) ,但成人变应性哮喘组T/T基因型患者血浆总IgE对数值为 2 6 4 90± 0 92 4 1与T/C基因型的 2 2 96 0± 1 10 4 0和C/C基因型的 2 3130± 0 80 5 2相比差异显著。②FcεRⅠβGlu2 37Gly位点Glu/Glu、Glu/Gly和Gly/Gly基因型频率为 0 5 6 6、0 377和 0 0 5 7,与正常对照相比差异显著 (χ2=7 31,P <0 0 5 ) ,Gly/Gly基因型血浆总IgE对数值为 2 72 2 0± 0 9374 ,与Glu/Glu和Glu/Gly相比差异显著。结论 :IgE高亲和力受体 β链启动区 - 10 9位T/T基因型与血浆总IgE高度相关 ,编码区 2

AIM: To investigate the linkage between the polymorphism of -109 and Glu237 in the high-affinity IgE receptorβ(FcεRⅠβ) gene and susceptibilty of allergic asthma in adults in a Chinese population. METHODS: Allergic asthma sample in adult and age-and sex-matched control were studied. A-109C/T and a coding variant Glu237Gly in FcεRⅠβ were detected with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: (1)The genotype frequencies were 0.415 for -109T/T, 0.491 for-109T/C and 0.094 for -109C/C in allergic asthma in adults. No significant difference in the distribution of-109C/T polymorphism was found between allergic asthma adult subjects and healthy control subjects. However, a homozygosity for the-109T allele was associated with increased total plasma IgE levels in subjects with allergic asthma (F=7.213, P<0.01). (2)The allele frequency of Gly237 in the patients and control was 0.245 and 0.142, respectively. There was a significant association between Gly/Gly genotype and allergic asthma in adults among allergic asthma patients. Gly237 was significantly associated with high IgE. CONCLUSIONS: It suggests that Gly237 vaviant of the FcεRⅠβ gene is involved in the development of allergic asthma. The-109C/T and Glu237Gly polymorphisms are two of the genetic factors identified, which (affects) total plasma IgE levels in adult allergic asthma in Han of Hubei province. [