东茛菪内酯对小鼠耐缺氧作用的研究

Anti-hypoxia activity of scopoletin in mice

目的:研究东茛菪内酯在小鼠常压条件下及脑缺血和心肌耗氧量增加的情况下耐缺氧的作用及其机制。方法:①常压耐缺氧实验法:小鼠(n=40)随机分为4组,分别腹腔注射东茛菪内酯(0.01mg/g,0.02mg/g),15min后,将小鼠分别放入250mL的磨口广口瓶内密封,以最后一次呼吸为指标,观察小鼠存活时间。②脑缺血实验法:小鼠(n=30)随机分为3组,分别腹腔注射东茛菪内酯(0.01mg/g,0.02mg/g),15min后,不需麻醉,用剪刀在耳下部快速断头,记录喘气时间。③异丙肾上腺素增加紧心肌耗氧量实验法:小鼠(n=30)随机分为3组,对照组按0.03mL/g剂量给小鼠皮下注射溶剂对照溶液,5min后按0.02mL/g剂量给小鼠腹腔注射溶剂对照溶液;模型组按0.015mg/g的剂量给小鼠皮下注射异丙肾上腺素,5min后按0.02mL/g剂量给小鼠腹腔注射溶剂对照溶液。给药组按0.015mg/g剂量给小鼠皮下注射异丙肾上腺素,5min后腹腔注射东茛菪内酯(0.01mg/g)。给药后10min,分别放入广口瓶内密封,记录小鼠存活时间。结果:东茛菪内酯(0.01mg/g,0.02mg/g)能非常显著延长常压耐缺氧条件下小鼠的存活时间犤(66.4±8.9)和(90.3±7.4)min犦,与对照组比较,差异均有显著性意义(t=3.356,3.147;P<0.01);东茛菪内酯(0.01mg/g,0.02mg/g)能显著延长断头小鼠的呼吸时间犤(21.2±0.8)和(23.5?

AIM:To study the effect and mechanism of scopoletin against hypoxia under the conditions of normal pressure, cerebral ischemia and the increase of myocardial oxygen consumption in mice.METHODS:①Experimental methods of normal pressure and anti-hypoxia:Forty mice were randomly divided into 4 groups,scopoletin was given by intraperitoneal injection(0.01 mg/g,0.02 mg/g),after 15 minutes,the mice were set into 250 mL jares which had milling ora,leaving each other and then airproofed,and the last breath was taken as the index,the living time of mice was observed.②Experimental methods of cerebral ischemia:Thirty mice were randomized into 3 groups,scopoletin was given by intraperitoneal injection(0.01 mg/g,0.02 mg/g),after 15 minutes,the heads of mice were cut down at the lower part of auris speedily,and the blowing time was noted.③Experimental methods of isoprenaline in increasing the myocardial oxygen consumption:Thirty mice were randomly divided into 3 groups,subcutaneous injection of normal saline(0.03 mL/g) was given to the mice in the control group,and then intraperitoneal injection of normal saline(0.02 mL/g) was performed after 5 minutes.The mice in the model group were treated with subcutaneous injection of isoprenaline(0.015 mg/g),and then with intraperitoneal injection of normal saline(0.02 mL/g) after 5 minutes.The mice in the scopoletin group received subcutaneous injection of isoprenaline(0.015 mL/g) to mice,and then intraperitoneal injection of scopoletin(0.01 mg/g) 5 minutes later.After 10 minutes,the mice were put into 250 mL jares,which had milling ora,leaving each other and then airproofed,the living time of the mice observed.RESULTS:Scopoletin(0.01 mg/g,0.02 mg/g) could very significantly prolong the living time[(66.4±8.9),(90.3±7.4) minutes] of mice under the condition of normal pressure and anti-hypoxia as compared with that in the control group(t=3.356,3.147;P< 0.01).Scopoletin(0.01 mg/g,0.02 mg/g) could also significantly prolong the respiratory time[(21.2 ±0.8),(23.5±0.7) s] of mice, whose heads were cut down(t=2.578,3.368;P< 0.05 to 0.01).Scopoletin(0.1 mg/10 g) could remarkably increase the survival time[(50.6±3.4) minutes] of mice under the condition of normal pressure and anti-hypoxia as compared with that in the control group(t=3.461;P< 0.01).CONCLUSION:Scopoletin has the effect of anti-hypoxia under the conditions of normal pressure,cerebral ischemia and the increase of myocardial oxygen consumption.