老年男性维生素D受体基因多态性与骨转换指标及1,25(OH)_2D_3的关系

Relationship of vitamin D receptor gene polymorphisms with bone turnover markers and 1,25-dihydroxy vitamin D_3 in elderly men

目的:维生素D受体(vitaminDreceptor,VDR)基因多态性已成为骨质疏松遗传学研究的热点,了解VDR基因多态性在中国老年男性中的分布,并进一步认识其与骨钙素及双羟维生素D3犤1,25(OH)2D3犦等的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,分析145例老年男性的VDR基因型,同时采用放免法测定血清骨钙素(bonegla-containingprotein,BGP)、I型胶原前胶原氨基端前肽(N-terminalpropeptideoftypeprocollagen,PINP)及1,25(OH)2D3。结果:VDR基因型分布频率为BB,0.014;Bb,0.117;bb,0.869。B,b等位基因分别占7.2%,92.8%。骨质疏松症的发生率为38.6%。骨质疏松组与非骨质疏松组之间VDR基因型分布频率的差异无显著性意义(P>0.05)。未发现VDR基因型与BGP,PINP及1,25(OH)2D3相关。结论:老年男性VDR基因型与骨钙素等骨转换升华指标及1,25(OH)2D3无明显联系。

AIM:Vitamin D receptor(VDR) gene polymorphism has been the focus of the study on the genetics of osteoporosis.The aim of this study is to investigate the frequency distribution of VDR gene polymorphism in Chinese elderly men,and further study the relationship of VDR gene polymorphism with bone gla-containing protein(BGP) as well as 1,25-dihydroxy vitamin D3[1,25(OH)2D3].METHODS:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to determine the VDR genotypes in 145 elderly men, serum BGP,collagen type I N-terminal propeptide as well as 1,25(OH)2D3 were determined by radioimmunoassay.RESULTS:The frequency distributions of the VDR genotypes of BB,Bb,bb were 0.014,0.117,0.869 respectively.The proportions of allele B and A were 7.2%and 92.8%respectively.The prevalence rate of osteoporosis was 38.6%.No association of VDR genotypes with the serum BGP,PINP and 1,25(OH)2D3 was observed.CONCLUSION:VDR genotype has no obvious correlation with the bone turnover markers (such as BGP) and 1,25(OH)2D3.